大豆GmSDG25基因的克隆及拟南芥遗传转化  

Cloning of Soybean GmSDG25 Gene and Genetic Transformation of Arabidopsis

作　　者：姜玲 阳小凤[1] 黄山[1] 唐文军 李小红[1] 马淑梅[1] Jiang Ling;Yang Xiaofeng;Huang Shan;Tang Wenjun;Li Xiaohong;Ma Shumei(Crop Research Institute of Hunan Province,Changsha,410125)

机构地区：[1]湖南省作物研究所,长沙410125

出　　处：《分子植物育种》2024年第16期5326-5334,共9页Molecular Plant Breeding

基　　金：湖南省农业科技创新资金项目(2020CX31)资助。

摘　　要：GmSDG25是拟南芥组蛋白赖氨酸甲基转移酶基因SDG25在大豆中的同源基因,为探究该基因的功能,本研究从栽培大豆品种‘湘春2701’中克隆到2个GmSDG25基因,分别命名为GmSDG25a和GmSDG25b,两者的全长cDNA长度分别是3684和3681 bp。通过构建过表达载体pFGC5941-35S::GmSDG25a/GmSDG25b,将其分别转化至拟南芥sdg25突变体中,对获得的T_(3)代纯合转基因拟南芥植株进行表型鉴定和分子检测,发现该转基因植株sdg25-35S::GmSDG25a和sdg25-35S::GmSDG25b的开花时间均表现出晚于sdg25突变体,接近于野生型(Col)开花的表型;并且植株内与开花调控相关的FLC、FT、SOC1基因的表达量较之Col无明显差异,说明GmSDG25a和GmSDG25b基因在拟南芥sdg25突变体中异位表达,能够互补sdg25的早花表型,使其恢复至Col的开花表型,表明GmSDG25a和GmSDG25b基因可能具有与拟南芥SDG25相似的调控植物开花的生物学功能。研究结果将为后续揭示GmSDG25基因参与大豆开花时间调控的作用机制提供理论参考。GmSDG25 is the homologous gene of Arabidopsis histone lysine methyltransferase gene SDG25 in soybean.In order to explore the function of this gene,we cloned two GmSDG25 genes from the soybean cultivar‘xiangchun 2701’,separately named as GmSDG25a and GmSDG25b,which of the full-length cDNA are 3684bp and 3681bp,respectively.The overexpression vectors pFGC5941-35S::GmSDG25a/GmSDG25b were constructed and transformed into the Arabidopsis mutant sdg25,respectively.The homozygous transgenic Arabidopsis plants of T3 generation were obtained and subjected to phenotype identification and molecular detection.It was found that the transgenic plants sdg25-35S::GmSDG25a and sdg25-35S::GmSDG25b exhibited flowering later than the sdg25 mutants and at the same time as the wild-type(col)plants.And compared with col,the expression of flowering-regulated genes FLC,FT,and SOC1 in the transgenic plants has no significant difference.These results indicated that both GmSDG25a and GmSDG25b could rescue the early-flowering phenotype of Arabidopsis sdg25 mutant,and restore it to the flowering phenotype of col,suggesting that GmSDG25a and GmSDG25b genes may have similar biological functions of regulating plant flowering as Arabidopsis SDG25.The results of this study will provide a theoretical reference for further revealing of the mechanism of GmSDG25 involved in the regulation of flowering time in soybean.

关 键 词：大豆 GmSDG25基因 基因克隆 遗传转化 

分 类 号：S565.1[农业科学—作物学]