过表达MeERF72对C3木薯淀粉合成的影响  

Effect of Overexpression of MeERF72 on Synthesis of C3 Cassava Starch

作　　者：廖明馨 李可[1] 李梦桃 鲍茹雪 陈新[2] 王文泉 Liao Mingxin;Li Ke;Li Mengtao;Bao Ruxue;Chen Xin;WangWenquan(College of Tropical Crops,Hainan University,Haikou,570228;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101)

机构地区：[1]海南大学热带作物学院,海口570228 [2]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《分子植物育种》2024年第16期5335-5341,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31671748)资助。

摘　　要：木薯蔗糖合酶1(MeSuSy1)是淀粉生物合成通路上第一个关键酶,而乙烯响应因子MeERF72可调控其转录活性。为探究MeERF72对木薯(Manihot esculenta Crantz)块根淀粉合成的影响,本研究在盆栽条件下,检测野生型、MeERF72过表达系和RNAi干扰系MeERF72和MeSuSy1在叶片和块根中的表达量,并测定木薯块根薯肉的淀粉、还原糖和总糖含量。结果显示,过表达株系的块根中MeERF72的表达量显著高于野生型C3,其MeSuSy1的表达量显著低于野生型C3,干扰植株反之;MeERF72干扰株系薯肉的淀粉和总糖含量显著高于野生型C3,过表达株系反之,表明MeERF72可能负调控MeSuSy1的转录活性,降低蔗糖分解效率,进而减少淀粉合成。本研究明确了MeERF72对MeSuSy1的调控机制和对木薯淀粉合成的影响,为进一步研究淀粉代谢通路的基因调控网络以及热带作物重要性状的遗传改良策略提供参考。Cassava sucrose synthase 1(MeSuSy1)is the first key enzyme in the starch biosynthesis pathway,and ethylene response factor MeERF72 regulates its transcriptional activity.In order to investigate the effect of Me-ERF72 on the synthesis of starch in cassava(Manihot esculenta Crantz)root tubers,the expression levels of MeERF72 and MeSuSy1 in leaves and tubers of wild type,MeERF72 overexpression lines and RNAi interfering lines were detected in pots,and the contents of starch,reducing sugar and total sugar in cassava root tubers were measured.The results showed that the expression level of MeERF72 in root tubers of overexpressed strains was significantly higher than that of wild-type C3,and the expression level of MeSuSy1 in root tubers of overexpressed strains was significantly lower than that of wild-type C3,versa for interference strain;the content of starch and total sugar in potato meat of MeERF72 interference strain was significantly higher than that of wild-type C3,versa for overexpression.These indicated that MeERF72 may negatively regulate the transcriptional activity of MeSuSy1,reduce sucrose decomposition efficiency,thus reduce starch synthesis.This study clarifies the regulatory mechanism of MeERF72 on MeSuSy1 and its effect on cassava starch synthesis,which will provide a reference for further research on the gene regulatory network of starch metabolic pathways and genetic improvement strategies for important traits in tropical crops.

关 键 词：木薯(Manihot esculenta Crantz) 淀粉生物合成 ERF基因 蔗糖合成酶 

分 类 号：S533[农业科学—作物学]