番茄病毒病种类及主要鉴定方法研究进展  

Research Progress on Tomato Virus Disease Species and MainIdentification Methods

作　　者：严婉荣[1] 王宝 吉训聪[1] 肖彤斌 林珠凤[1] 王会芳[1] 曾向萍 赵志祥[1] Yan Wanrong;Wang Bao;Ji Xuncong;Xiao Tongbin;Lin Zhufeng;Wang Huifang;Zeng Xiangping;Zhao Zhixiang(Scientific Observation and Experiment Station of Crop Pests in Haikou(Ministry of Agriculture),Key Laboratory of Plant Diseases and Pest Control of Hainan Province,Research Center of Quality Safety and Standards for Agro-Products,Hainan Academy of Agricultural Sciences,Institute of Plant Protection,Hainan Academy of Agricultural Sciences,Haikou,571100)

机构地区：[1]海南省农业科学院植物保护研究所(海南省农业科学院农产品质量安全与标准研究中心),海南省植物病虫害防控重点实验室,农业农村部海口作物有害生物科学观测实验站,海口571100

出　　处：《分子植物育种》2024年第16期5357-5367,共11页Molecular Plant Breeding

基　　金：海南省省属科研院所技术创新专项(jscx202013);海南省重大科技计划项目(ZDKJ2021007);海南省重点研发计划项目(ZDYF2020055);海南省财政科技计划项目(SQKY2022-004)共同资助。

摘　　要：番茄病毒病是影响番茄产量和品质的重要因素之一。为了明确近几年国内外番茄病毒病发生动态,本研究对2012—2022年以来,世界各地首次报道的番茄病毒病新种类、地区新记录进行了统计,对统计到的22种病毒从发现的时间地点、分布范围、传播方式及症状进行了阐述。明确了侵染中国番茄的病毒数量和种类,将中国报道的番茄病毒病种类由35种更新至44种。本研究从番茄病毒病的多种鉴定方法,针对一些常见病毒和新型病毒,提供了全面、快速的RT-PCR(PCR)检测方法。预测了可能会对中国番茄产业造成入侵危害的新病毒。为番茄病毒病的发生、发展和检测提供了最新数据,为做好番茄病毒病的全面监测和防控提供理论参考。Tomato virus disease is one of the most important factors affecting tomato yield and quality.In order to clarify the occurrence trend of tomato virus disease at home and abroad in recent years,in this research,we counted the new species and regional records of tomato virus diseases were reported for the first time in the world since 2012-2022.The 22 viruses counted are described in terms of where and when they were found,their distribution,spread and symptoms.The number and species of viruses infecting tomato in China were determined,and the number of reported tomato virus disease species in China was updated from 35 to 44.A variety for identification methods of tomato virus diseases were reviewed.A comprehensive and rapid RT-PCR(PCR)method was provided for the detection of some common viruses and new types of viruses.The new viruses that may cause invasion harm to the tomato industry in China were predicted.This paper provides the latest data for the occurrence,development and detection of tomato virus disease,and a provides theoretical reference for the comprehensive monitoring and control of tomato virus disease.

关 键 词：番茄病毒病 新病毒 鉴定方法 RT-PCR检测 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治]