超高效液相色谱-四极杆-飞行时间质谱分析匙羹藤化学成分  

Analysis of chemical constituents in Gymnema sylvestre(Retz.)Schult via ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry

作　　者：曾志浩 肖观林 贾灿潮 江洁怡 汤瑞茵 贾德政 李养学 李绍林[4] 毕晓黎[1,2,3] ZENG Zhi-hao;XIAO Guan-lin;JIA Can-chao;JIANG Jie-yi;TANG Rui-yin;JIA De-zheng;LI Yang-xue;LI Shao-lin;BI Xiao-li(The Fifth Clinical College of Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Guangdong Province Engineering Technology Research Institute of Traditional Chinese Medicine,Guangzhou 510095,China;Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine,Guangzhou 510095,China;Guangdong Food and Drug Vocational College,Guangzhou 510520,China)

机构地区：[1]广州中医药大学第五临床医学院,广州510405 [2]广东省中医药工程技术研究院,广州510095 [3]广东省中医药研究开发重点实验室,广州510095 [4]广东食品药品职业学院,广州510520

出　　处：《中国新药杂志》2024年第15期1595-1607,共13页Chinese Journal of New Drugs

基　　金：广东省科技创新战略专项(部省市联动专项与省属科研机构稳定性支持)资助项目(粤财科教[2021]113号);广州市科技计划资助项目(202102080588)。

摘　　要：目的:基于超高效液相色谱-四极杆-飞行时间质谱(ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry,UPLC-Q-TOF-MS/MS)技术分析匙羹藤的化学成分。方法:采用Waters CORTECS UPLC T_(3)色谱柱(150 mm×2.1 mm,1.6μm)以0.1%甲酸-乙腈的流动相梯度洗脱,电喷雾负离子模式下对色谱流出物进行检测,检测范围m/z 50~2000。结果:通过高分辨质谱数据(一级质谱及二级质谱碎片)分析,结合数据库、文献以及对照品比对,共鉴别出101种成分,包括53种三萜类、37种黄酮类、5种有机酸类、3种单萜类、1种二萜类、1种菲类、1种甾体类化合物。结论:本研究通过UPLC-Q-TOF-MS/MS技术快速鉴别了匙羹藤的化学成分,该方法快速、准确、简便,能够用于匙羹藤化学成分的定性鉴定,为该草药的质量标准以及物质基础研究奠定基础。Objective:Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)was used to analyze the chemical constituents of Gymnema sylvestre(Retz.)Schult.Methods:A Waters CORTECS UPLC T_(3)(150 mm×2.1 mm,1.6μm)column was utilized to separate the components using the mobile phase at a gradient elution of 0.1%formic acid-acetonitrile and the effluent was detected in electrospray anionic mode with the detection range of m/z 50~2000.Results:Combined with the databases,literature,and reference standards,we identified 101 components,including 53 triterpenoids,37 flavonoids,5 organic acids,3 monoterpenoids,1 diterpenoid,1 phenanthrene,and 1 steroid based on the analysis of high-resolution mass spectrometry data(primary mass spectra and secondary mass spectral fragments).Conclusion:The chemical constituents of Gymnema sylvestre were identified using the fast,accurate,and simple UPLC-Q-TOF-MS/MS method for qualitative identification,which laid the foundation for establishing a quality standard of the herb and its material basis research.

关 键 词：匙羹藤 超高效液相色谱-四极杆-飞行时间质谱 化学成分 三萜类化合物 黄酮类化合物 

分 类 号：R917[医药卫生—药物分析学]