NiFe_(2)O_(4)磁性纳米粒子制备及其吸附富组氨酸蛋白的研究  

Preparation of NiFe_(2)O_(4) Magnetic Nanoparticles for Affinity Adsorption of Histidine-Rich Proteins

作　　者：佟彤 刘思齐 单海霞 李海彦 TONG Tong;LIU Siqi;SHAN Haixia;LI Haiyan(School of Life Science and Technology,Changchun University of Science and Technology,Changchun 130022;School of Physics,Changchun University of Science and Technology,Changchun 130022;Jilin University Press,Changchun 130021)

机构地区：[1]长春理工大学生命科学技术学院,长春130022 [2]长春理工大学物理学院,长春130022 [3]吉林大学出版社,长春130021

出　　处：《长春理工大学学报（自然科学版）》2024年第4期107-114,共8页Journal of Changchun University of Science and Technology(Natural Science Edition)

基　　金：吉林省科技发展计划项目(20230204090YY)。

摘　　要：引入具有生物相容性、亲水性好的高分子材料聚乙二醇,通过水热法一步合成NiFe_(2)O_(4)磁性纳米材料,其直径大小约为50 nm,比表面积38.15 m^(2)·g^(-1),具有良好的结晶度、热稳定性、分散性以及较强的磁性。利用NiFe_(2)O_(4)磁性纳米材料NiFe_(2)O_(4)MNPs表面的Ni2+能够与富组氨酸蛋白上的咪唑环通过配位键结合,以此来实现对富组氨酸蛋白的吸附。实验证明NiFe_(2)O_(4)磁性纳米材料能吸附天然富组氨酸蛋白质牛血红蛋白,吸附量可达到235 mg·g^(-1),高于文献报道的120.1 mg·g^(-1),还可以用于人血血红蛋白的吸附,具有实际应用价值。Introducing biocompatible and hydrophilic polymer material polyethylene glycol,NiFe_(2)O_(4magnetic) nanomaterials were synthesized via a one-step hydrothermal method.The average diameter size of the nanomaterial was 50 nm and it had a specific surface area of 38.15 m^(2)·g^(-1)with good crystallinity,thermal stability,dispersion and strong magnetic properties.Ni2+on the surface of NiFe_(2)O_(4)MNPs could form coordination bonds with the imidazole ring of histidine-rich proteins,so as to bind with histidine-rich proteins.The results showed that NiFe_(2)O_(4)magnetic nanomaterial could bind with natural histidine-rich protein bovine hemoglobin,the binding capacity was 235 mg·g^(-1),which was higher than 120.1 mg·g^(-1)reported in the literature.It could also be used for binding with hemoglobin in human blood,which has good practical application value.

关 键 词：蛋白质吸附 磁性纳米材料 富组氨酸蛋白质 双金属氧化物 

分 类 号：TB383[一般工业技术—材料科学与工程]