微塑料对生物除磷工艺的影响及微生物学机制探究  

Effect of Microplastics on Biological Phosphorus Removal Process and Its Microbiological Mechanism

作　　者：王宏涛 姜楠 李洋 雷恒[1,2] WANG Hongtao;JIANG Nan;LI Yang;LEI Heng(Yellow River Conservancy Technical Institute,Kaifeng 475004,China;Henan Engineering Research Center of Prject Operation and Eeological Security for Inter-Basin Regional Water Diversion Project,Kaifeng 475004,China;Henan Hydrology and Water Resources Center,Zhengzhou 450003,China)

机构地区：[1]黄河水利职业技术学院,河南开封475004 [2]河南省跨流域区域引调水运行与生态安全工程研究中心,河南开封475004 [3]河南省水文水资源中心,河南郑州450003

出　　处：《水处理技术》2024年第8期75-80,共6页Technology of Water Treatment

基　　金：河南省重大科技专项项目(231100320100);国家级职业教育教师教学创新团队课题研究项目(ZH2021040101);河南省2023年水利科技攻关项目计划(GG202337);开封市科技攻关计划(2103028)。

摘　　要：微塑料(MPs)是生活废水内常被检出的新污染物,然而关于MPs如何影响强化生物除磷工艺及相关机制的研究较少。因此,本工作构建了序批式EBPR工艺,通过控制进水聚苯乙烯微塑料浓度,考察了MPs对EBPR的影响并揭示相关机制。结果表明,MPs对EBPR具有剂量效应,低于0.3 mg/LMPs对EBPR性能及污泥特征影响不明显,而超过3.0 mg/LMPs降低了EBPR对污染物和营养盐的去除,并降低污泥浓度,提高胞外聚合物含量。在10.0 mg/L MPs组别内,COD、NH_(4)^(+)-N和总磷(TP)去除率分别下降至84.6%~89.5%、84.9%~89.6%和74.6%~77.8%,污泥浓度下降至4.08~4.16 g/L,但EPS含量增加至123.6~126.5 mg/g。机制分析表明MPs抑制了厌氧阶段TP的释放及好氧阶段TP的吸收。高浓度MPs降低了与污染物和营养盐去除相关关键酶的活性。MPs降低了微生物Proteobacteria和Chloroflexi门级别的相对丰度。研究结果为EBPR工艺处理含MPs废水提供一定的数据支撑。Microplastics (MPs) are a new pollutant often detected in domestic wastewater,but there are few studies on how MPs affect the enhanced biological phosphorus removal process and related mechanisms.Therefore,the sequencing batch EBPR process was constructed in this work.By controlling the concentration of influent polystyrene microplastics,the effect of MPs on EBPR was investigated and the related mechanism was revealed.The results showed that MPs had a dose effect on EBPR,while less than 0.3 mg/L MPs had no obvious effect on EBPR performance and sludge characteristics,while more than 3.0 mg/L MPs reduced the removal of pollutants and nutrients by EBPR,decreased the sludge concentration and increased the extracellular polymer content.In the 10.0 mg/L MPs group,the removal efficiency of COD,NH_4~+-N and total phosphorus (TP)decreased to 84.6%~89.5%,84.9%~89.6%and 74.6%~77.8%,respectively,and the sludge concentration decreased to 4.08~4.16 g/L,but the EPS content increased to 123.6~126.5 mg/g.Mechanism analysis showed that MPs inhibited the release of TP in the anaerobic phase and the absorption of TP in the aerobic phase.High concentrations of MPs decreased the activities of key enzymes related to the removal of pollutants and nutrients.MPs decreased the relative abundance of microbial Proteobacteria and Chloroflexi.The research results provide some data support for the treatment of wastewater containing MPs by the EBPR process.

关 键 词：EBPR 微塑料 污泥特征 关键酶活性 微生物 

分 类 号：O643.36[理学—物理化学] X703[理学—化学]