晚期糖基化终末产物受体在波动性高糖致人冠状动脉内皮细胞凋亡中的作用  

The role of the receptor of advanced glycosylation end products in apoptosis of human coronary endothelial cells induced by intermittent high glucose

作　　者：庄微[1] 孔娜娜 刘挺松 王磊[1] ZHUANG Wei;KONG Nana;LIU Tingsong;WANG Lei(Department of Cardiovascular Medicine,Jinling Clinical Medical College,Nanjing University of Chinese Medicine/General Hospital of Eastern Theater Command,PLA,Nanjing 210002,Jiangsu,China)

机构地区：[1]南京中医药大学金陵临床医学院(东部战区总医院)心血管内科,南京210002

出　　处：《医学研究与战创伤救治》2024年第5期482-487,共6页Journal of Medical Research & Combat Trauma Care

基　　金：东部战区总医院院内课题(YYQN2021092)。

摘　　要：目的研究持续性高糖(CHG)和波动性高糖(IHG)环境下,人冠状动脉内皮细胞(HCAECs)的细胞凋亡情况,并探索HCAECs细胞凋亡是否由晚期糖基化终末产物受体(RAGE)介导发生。方法取对数生长期的HCAECs分为4组:①正常血糖组(CNG组,5 mmol/L葡萄糖);②持续性高糖组(CHG组,20 mmol/L葡萄糖);③波动性高糖组(IHG组,5 mmol/L和20 mmol/L葡萄糖每8 h波动1次);④波动性甘露醇对照组(IM组,5 mmol/L和20 mmol/L甘露醇每8小时波动1次,保持与波动性高糖相同的波动性渗透压环境)。不同条件处理后的HCAECs,采用噻唑蓝(MTT)法测定细胞活力,流式细胞术检测HCAECs细胞凋亡情况,Westernblot检测Caspase-3、RAGE的蛋白表达水平变化,qRT-PCR检测RAGE的转录水平变化情况。用靶向RAGE的shRNA慢病毒敲低HCAECs中RAGE的表达量,观察IHG中Caspase-3的活化表达情况。结果与CNG组相比,CHG组和IHG组细胞活力均降低(P<0.05),但IHG组细胞活力更低(P=0.035);IHG组细胞凋亡率增加(P=0.028),Caspase-3活化状态(P=0.037)和RAGE表达量显著增加(P<0.05)。用靶向RAGE的shRNA慢病毒敲低RAGE表达水平,发现波动性高糖导致的细胞凋亡情况得到明显改善(P<0.05)。结论波动性高糖可通过增强RAGE表达,促进细胞凋亡,引起HCAECs损伤。Objective We found that intermittent high glucose in diabetic patients was easy to suffer from coronary atherosclerosis.To explore the mechanism,we investigated the apoptosis of human coronary endothelial cells in the context of persistent and fluctuating hyperglycemia,and explored whether apoptosis was mediated by the receptor of advanced glycosylation end products.Methods The HCAECs of logarithmic growth period were divided into 4 groups:①normal blood glucose group(CNG,5mmol/L glucose);②constant high glucose group(CHG,20 mmol/L glucose);③intermittent high glucose group(IHG,5mmol/L and 20 mmol/L glucose fluctuated every 8 h);④intermittent mannitol control group(IM,5 mmol/L and 20 mmol/L mannitol fluctuated every 8 h,main-taining the same fluctuating osmotic pressure environment as intermittent high glucose).HCAECs treated under different conditions were selected.Cell viability was determined by MTT assays.The apoptosis of HCAECs was detected by flow cytometry.Protein levels of Caspase-3 and RAGE were detected by Western blotting,and transcription levels of RAGE were detected by qRT-PCR.The expression quantity of RAGE in HCAECs was knockdown by shRNA using lentivirus trans-fection.The expression of Caspase-3 in IHG was observed.Results Compared with the CNG group,the cell viability of the constant high glucose group(CHG)and the intermittent high glucose group(IHG)was decreased(P<0.05),but the cell viability of the IHG group was lower(P=0.035).The apoptosis rate(P=0.028),Caspase-3 activation(P=0.037)and RAGE expression were significantly increased in IHG group(P<0.05).Finally,we knocked down the RAGE expression level and found that the apoptosis of HCAECs induced by intermittent high glucose was significantly improved(P<0.05).Conclusion Intermittent high glucose can promote apoptosis and damage HCAECs by enhancing RAGE expression.

关 键 词：波动性高糖 晚期糖基化终末产物受体 人冠状动脉内皮细胞 细胞凋亡 

分 类 号：R587.1[医药卫生—内分泌]