青春初期女性行卵巢组织冷冻过程中发现成熟卵母细胞一例  

Mature oocyte found during ovarian tissue cryopreservation in an early adolescent female

作　　者：张豪城 施丽冰 王海潮 竺海燕[1,2,3] ZHANG Haocheng;SHI Libing;WANG Haichao;ZHU Haiyan(Reproductive Department,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou 310016,China;Zhejiang Provincial Clinical Research Center for Obstetrics and Gynecology,Hangzhou 310016,China;Zhejiang Provincial Key Laboratory of Precise Protection and Promotion of Fertility,Hangzhou 310016,China)

机构地区：[1]浙江大学医学院附属邵逸夫医院生殖医学中心,浙江杭州310016 [2]浙江省妇产疾病临床医学研究中心,浙江杭州310016 [3]浙江省生育力精准保护与促进重点实验室,浙江杭州310016

出　　处：《浙江大学学报（医学版）》2024年第4期527-530,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省“尖兵领雁+X”研发公关计划(2024C03242)。

摘　　要：一例15岁霍奇金淋巴瘤患者行一个疗程化疗后来浙江大学医学院附属邵逸夫医院生殖中心进行生育力保存。在卵巢组织冷冻保存期间发现1枚MⅡ成熟卵母细胞和3枚生发泡期卵母细胞。3枚未成熟卵母细胞行体外成熟培养未获得成熟卵子,最终玻璃化冷冻1枚成熟卵母细胞和12片卵巢皮质。提示对于青春期性腺轴反馈已建立患者,卵巢组织冷冻不是唯一的生育力保存方式,可考虑促排卵取卵冻存卵母细胞,或根据月经周期判断处于排卵期者,在卵巢组织冷冻前尝试穿刺取卵获得自然周期成熟卵母细胞行卵母细胞冷冻,从而提高患者生育力保存概率。A 15-year-old female with Hodgkin’s lymphoma underwent ovarian tissue cryopreservation for preserving fertility in Reproductive Department of Sir Run Run Shaw Hospital,Zhejiang University School of Medical after receiving one course of chemotherapy.During the ovarian tissue cryopreservation,one MⅡmature oocyte and three germinal vesicle oocytes were found.The three immature oocytes underwent in vitro maturation but failed.Ultimately,one mature oocyte and 12 ovarian cortex slices were cryopreserved using vitrification.This case indicates that for patients with established gonadal axis feedback,ovarian tissue cryopreservation may not be the only method for fertility preservation.It is advisable to consider ovarian stimulation and oocyte retrieval for oocyte cryopreservation.Alternatively,for individuals in the ovulation phase of their menstrual cycle,attempting oocyte retrieval before ovarian tissue cryopreservation to obtain mature oocytes from the natural cycle,followed by oocyte cryopreservation,may enhance the likelihood of successful fertility preservation.

关 键 词：生育力保存 青春初期 卵巢 冷冻 卵子 体外成熟培养 病例报告 

分 类 号：R715[医药卫生—妇产科学]