乳酸酚棉兰染色在气传花粉鉴定中的应用  

Application of lactophenol cotton blue staining in identification of airborne pollens

作　　者：张续德[1] 冯少杰 刘菁菁 王昱琳 刘凤霞[1] 张芹 ZHANG Xude;FENG Shaojie;LIU Jingjing;WANG Yulin;LIU Fengxia;ZHANG Qin(Department of Allergy,Weifang People’s Hospital,Weifang,Shandong 261041,China;Weifang Meteorological Bureau,Weifang,Shandong 261011,China)

机构地区：[1]潍坊市人民医院过敏反应科,山东潍坊261041 [2]潍坊市气象局,山东潍坊261011

出　　处：《中华临床免疫和变态反应杂志》2024年第4期383-388,共6页Chinese Journal of Allergy & Clinical Immunology

基　　金：山东省医药卫生科技项目(202303111109);潍坊市卫健委计划项目(WFWSJK-2021-004,WFWSJK-2021-225);潍坊市科技发展计划项目(2023YX002)。

摘　　要：目的探索乳酸酚棉兰(lactophenol cotton blue,LPCB)染色方法在气传花粉镜检中的潜在应用价值。方法分别采用碱性复红(basic fuchsin,BF)染色与LPCB染色鉴定潍坊市春季、夏秋季主要气传花粉并记录每日花粉粒数。结果在鉴定构树花粉、悬铃木属花粉、蒿属花粉、苋科花粉和禾本科花粉方面,LPCB染色较BF染色更具优势,而在鉴定柏科花粉方面,BF染色更具优势。此外,潍坊市夏秋季优势花粉近40年无明显变化,由于绿化树种的改变,悬铃木属花粉已成为本地春季的主要致敏花粉。结论LPCB染色可同时用于真菌和气传花粉的鉴定,在保证鉴定准确性的同时,节约时间成本。Objective To explore the potential application of lactophenol cotton blue(LPCB)staining method in identification of airborne pollen under microscope.Methods The major airborne pollens of spring and autumn in Weifang City was identified by using basic fuchsin(BF)staining and LPCB staining and results of daily pollen count were recorded.Results The results of this study demonstrated that LPCB staining showed obvious advantages in identification of Broussonetia papyrifera,Platanus,Artemisia,Amaranthacae and Gramineae pollens,compared with BF staining.However,it showed disadvantage in identification of CUPRESSACEAE pollen.In addition,the major airborne pollens of summer and autumn have not significant difference within last 40 years in Weifang.Due to alternation of planting tree species,Platanus pollen has become the major allergenic pollen in spring.Conclusion LPCB staining can be used for identification of fungi and airborne pollens concurrently,which may ensure accuracy of identification as well as saving time costs.

关 键 词：气传花粉 镜检 碱性复红 乳酸酚棉兰 染色 

分 类 号：R122.1[医药卫生—环境卫生学]