薄荷醇对果蝇产卵驱避作用及毒杀效果  

Oviposition repellency and toxicity of menthol against Drosophila

作　　者：李迅 刘彤霄 李隆琦 李一璇 姜雅琦 陈利荣 刘威 LI Xun;LIU Tong-Xiao;LI Long-Qi;LI Yi-Xuan;JIANG Ya-Qi;CHEN Li-Rong;LIU Wei(College of Plant Protection,Anhui Agricultural University,Hefei 230036,China;Department of Clinical Medicine,Fenyang College,Shanxi Medical University,Fenyang 032200,China;Department of Laboratory Medicine,Fenyang College,Shanxi Medical University,Fenyang 032200,China)

机构地区：[1]安徽农业大学植物保护学院,合肥230036 [2]山西医科大学汾阳学院临床医学系,汾阳032200 [3]山西医科大学汾阳学院医学检验系,汾阳032200

出　　处：《昆虫学报》2024年第7期964-975,共12页Acta Entomologica Sinica

基　　金：山西省高等学校大学生创新创业训练项目(S202117114006)。

摘　　要：【目的】研究薄荷醇对常见几种果蝇Drosophila产卵的驱避作用,并通过后代存活率和发育历期及成虫死亡率以评价薄荷醇毒杀作用。【方法】应用产卵双向选择装置,在0.05%,0.1%和0.2%薄荷醇作用下检测果蝇的产卵选择性;利用双染料食物偏好实验检测薄荷醇对果蝇的摄食行为影响;利用产卵装置,检测薄荷醇对黑腹果蝇D.melanogaster的位置的影响;利用黑暗条件、摘除触角和前腿以及Orco 2突变体测定薄荷醇对黑腹果蝇产卵选择性的影响;并测定薄荷醇对黑腹果蝇后代存活率与发育历期的影响;测定薄荷醇对黑腹果蝇成虫寿命、奔跑速度和攀爬速度的影响;利用二氢乙锭(dihydroethidium,DHE)荧光染色技术,评估喂食1%薄荷醇3 d后黑腹果蝇成虫肠道内活性氧(reactive oxygen species,ROS)水平。【结果】薄荷醇对果蝇雌成虫产卵有驱避性,在0.05%,0.1%和0.2%薄荷醇作用下黑腹果蝇雌成虫的产卵指数分别为-0.42,-0.58和-0.98;铃木氏果蝇D.suzukii、雅库巴果蝇D.yakuba和拟暗果蝇D.pseudoobscura雌成虫对薄荷醇的产卵避性反应与黑腹果蝇雌成虫相同。在摄食过程中,黑腹果蝇及铃木氏果蝇成虫均对薄荷醇培养基产生明显排斥反应。薄荷醇培养基对黑腹果蝇成虫有明显的位置驱避性,0.05%,0.1%和0.2%薄荷醇处理下位置指数分别为-0.32,-0.44和-0.84。各个浓度的薄荷醇对黑暗环境和摘除前足的黑腹果蝇雌成虫仍然表现出显著的产卵驱避性,但在摘除黑腹果蝇成虫触角的条件下,薄荷醇对它们的产卵驱避性显著降低,0.05%,0.1%和0.2%薄荷醇作用下产卵指数分别为-0.04,-0.06和-0.26,薄荷醇对Orco 2突变体仍旧有产卵驱避性。与对照组相比,1%薄荷醇处理组黑腹果蝇成蛹和成虫羽化时间明显延长,分别延长了0.74和0.73 d,存活率明显下降,分别下降了63.2%和91.3%。随着薄荷醇浓度的增加黑腹果蝇成虫存活率呈现下降的趋势,且明【Aim】To study the repellent effects of menthol on the oviposition of several common Drosophila species,and to evaluate the toxic effect of menthol through the survival rate and developmental duration of offspring and the mortality rate of adult Drosophila.【Methods】The oviposition selection of Drosophila was assessed by using the two-choice apparatus under the action of 0.05%,0.1%and 0.2%menthol.The double dye food preference experiment was used to detect the effects of menthol on the feeding behavior of Drosophila.The effects of menthol on the location of D.melanogaster was tested by oviposition apparatus.The effects of menthol on the oviposition selection of D.melanogaster were investigated by using dark conditions,removal of antennae and forelegs,and Orco 2 mutant test.The effects of menthol on the survival rate and developmental duration of D.melanogaster offspring were investigated.The effects of menthol on the lifespan,running speed and climbing speed of adult D.melanogaster were detected.The reactive oxygen species(ROS)level in the gut of D.melanogaster adults fed with 1%menthol for 3 d was evaluated by dihydroethidium(DHE)fluorescence staining technique.【Results】Menthol repelled the oviposition of female adults of Drosophila,and the oviposition indexes of D.melanogaster were-0.42,-0.58 and-0.98 under the treatment of 0.05%,0.1%and 0.2%menthol,respectively.The female adults of D.suzukii,D.yakuba and D.pseudoobscura also showed oviposition avoidance response to menthol in the same way as those of D.melanogaster.D.melanogaster and D.suzukii adults significantly rejected the medium containing menthol during the feeding process.D.melanogaster adults showed obvious positioning avoidance to the medium containing menthol,and the position indexes of D.melanogaster under the treatment of 0.05%,0.1%and 0.2%menthol were-0.32,-0.44 and-0.84,respectively.All concentrations of menthol still showed significant oviposition repellency to the female adults of D.melanogaster in dark environment and with foreleg re

关 键 词：果蝇 薄荷醇 产卵避性 嗅觉系统 发育历期 存活率 

分 类 号：Q965.8[生物学—昆虫学]