冠心病患者血清miR-3129-5p和miR-6870-3p表达与疾病严重程度的关系研究  

Relationship Between Serum mir-3129-5p and mir-6870-3p Expression and Disease Severity in Patients with Coronary Heart Disease

作　　者：邵桂丽[1] 武琼[1] 王梦娟[2] 王芳[3] Shao Guili;Wu Qiong;Wang Mengjuan;Wang Fang(Department of Cardiology,The Affiliated Cardiovascular Hospital of Qingdao University,Qingdao 266000,China;Department of Emergency,Qingdao Municipal Hospital,Qingdao 266071,China;Department of Cardiovascular,Qingdao Third People's Hospital,Qingdao 266041,China)

机构地区：[1]青岛大学附属心血管病医院心内科,青岛266000 [2]青岛市市立医院急诊内科,青岛266071 [3]青岛市第三人民医院心血管内一科,青岛266041

出　　处：《成都医学院学报》2024年第4期628-632,共5页Journal of Chengdu Medical College

基　　金：山东省中医药管理局科技项目(No:2021M165)。

摘　　要：目的探究冠心病(CHD)患者血清miR-3129-5p和miR-6870-3p表达与疾病严重程度的关系。方法以2021年3月至2023年7月在青岛大学附属心血管病医院进行治疗的80例CHD患者为研究对象(CHD组),根据Gensini评分将CHD患者分为轻度组(n=27)、中度组(n=33)和重度组(n=20),另选取同期在本院进行体检的健康者80例为对照组。实时荧光定量PCR(qRT-PCR)法测定血清miR-3129-5p、miR-6870-3p水平;血清miR-3129-5p、miR-6870-3p水平与Gensini评分之间的关系采用Spearman相关性分析;发生CHD的影响因素采用Logistic多因素模型分析;血清miR-3129-5p、miR-6870-3p水平对CHD的诊断价值采用受试者工作特征(ROC)曲线分析。结果CHD组患者血清miR-3129-5p、miR-6870-3p水平均高于对照组(P<0.05),且随着病情程度的增加,CHD患者血清miR-3129-5p、miR-6870-3p水平逐渐升高(P<0.05)。Spearman相关性分析结果显示,血清miR-3129-5p、miR-6870-3p水平与Gensini评分均呈正相关(r_(1)=0.411,r_(2)=0.431,P<0.001)。Logistic回归分析结果显示,总胆固醇(TC)、低密度脂蛋白(LDL-C)、甘油三酯(TG)、miR-3129-5p、miR-6870-3p升高均为发生CHD的危险因素(P<0.05),高密度脂蛋白(HDL-C)升高为发生CHD的保护因素(P<0.05)。ROC曲线结果显示,血清miR-3129-5p、miR-6870-3p诊断CHD的曲线下面积(AUC)分别为0.855、0.852,敏感度分别为78.8%、83.8%,特异度分别为65.1%、56.3%,二者联合预测CHD的AUC为0.927,敏感度为77.5%、特异度为73.8%。结论CHD患者血清miR-3129-5p、miR-6870-3p水平均升高,且血清miR-3129-5p、miR-6870-3p与CHD患者疾病严重程度密切相关。miR-3129-5p、miR-6870-3p联合检测可作为诊断CHD的重要辅助参考指标。Objective To investigate the relationship between the serum expression of miR-3129-5p and miR-6870-3p and the severity of disease in patients with coronary heart disease(CHD).Methods A total of 80 patients with CHD treated in the Affiliated Cardiovascular Hospital of Qingdao University from March 2021 to July 2023 were selected as the study objects(CHD group).According to the Gensini score,the CHD patients were further grouped into mild group(n=27),moderate group(n=33),and severe group(n=20).And 80 healthy individuals who underwent physical examination in the hospital during the same time were selected as the control group.Real-time fluorescence quantitative PCR(qRT-PCR)was applied to determine the serum levels of miR-3129-5p and miR-6870-3p.Spearman correlation was applied to analyze the relationship between serum miR-3129-5p and miR-6870-3p levels and Gensini score.Logistic multivariate model was used to analyze the influencing factors of CHD.ROC curve was applied to analyze the diagnostic value of serum miR-3129-5p and miR-6870-3p levels for CHD.Results The serum levels of miR-3129-5p and miR-6870-3p in the CHD group were higher than those in the control group(P<0.05).And the serum levels of mir-3129-5p and mir-6870-3p in patients with CHD increased gradually with the increase of disease severity(P<0.05).Spearman correlation analysis showed that serum levels of miR-3129-5p and miR-6870-3p were positively correlated with the Gensini score(r_(1)=0.411,r_(2)=0.431,P<0.001).Logistic regression analysis results showed that the increase of total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),triacylglyceride(TG),miR-3129-5p and miR-6870-3p were risk factors for the development of CHD(P<0.05),and the increase of high-density lipoprotein cholesterol(HDL-C)was a protective factor for the development of CHD(P<0.05).ROC curve results showed that the AUC of serum miR-3129-5p and miR-6870-3p in diagnosing CHD was 0.855 and 0.852,respectively,with sensitivity of 78.8%and 83.8%,specificity of 65.1%and 56.3%,respe

关 键 词：冠心病 miR-3129-5p miR-6870-3p 疾病严重程度 相关性 

分 类 号：R541.4[医药卫生—心血管疾病]