黄连提取液对多重耐药鲍曼不动杆菌的抑菌作用及对其生物膜的影响  

Antibacterial effect of Coptis chinensis extract on multidrug-resistant Acinetobacter baumannii and its effect on biofilm

作　　者：余艳妮 覃艳春[2] 蓝怡 崔文卿 葛珍珍 何一恒 李笑 杨珊[2] YU Yanni;QIN Yanchun;LAN Yi;CUI Wenqing;GE Zhenzhen;HE Yiheng;LI Xiao;YANG Shan(Youjiang Medical University for Nationalities,Baise,Guangxi 533000,China;Department of Pathogenic biology and Immunology,Youjiang Medical University for Nationalities,Baise,Guangxi 533000,China)

机构地区：[1]右江民族医学院,广西百色533000 [2]右江民族医学院病原生物学与免疫学教研室,广西百色533000

出　　处：《现代医药卫生》2024年第16期2708-2712,2718,共6页Journal of Modern Medicine & Health

基　　金：右江民族科学技术局医学院校级科研基金项目(yy2020ky025);大学生创新创业训练计划项目(S202110599094,S202210599107);百色市科学研究与技术开发计划项目(20203403)。

摘　　要：目的探讨黄连提取液对多重耐药鲍曼不动杆菌(MDR-Ab)抑菌作用及对其细胞膜的影响,为黄连提取液治疗MDR-Ab提供理论依据。方法2023年5月采用牛津杯法测定黄连提取液对MDR-Ab的最低抑菌浓度(MIC),扫描电镜观察黄连提取液对MDR-Ab菌体结构的破坏,碘化丙啶染色法观察黄连提取液对MDR-Ab生物膜的作用,检测黄连提取液对MDR-Ab作用后细胞内外三磷酸腺苷(ATP)含量。结果黄连提取液对MDR-Ab的MIC为64 mg/mL。黄连提取液处理的MDR-Ab菌体形态发生凹陷、扭曲等改变,大部分染成红色,且其形态改变程度、染成红色比例均与黄连提取液浓度呈正比。MDR-Ab细胞内ATP含量随黄连提取液浓度增高逐渐减少,细胞外ATP含量随黄连提取液浓度增高逐渐增多。结论黄连提取液对MDR-Ab具有抑菌作用,且通过破坏MDR-Ab细胞膜发挥抑菌作用。Objective To investigate the antibacterial effect of Coptis chinensis extract on multidrug-resistant Acinetobacter baumannii(MDR-Ab)and its effect on cell membrane,and to provide theoretical basis for the treatment of MDR-Ab by Coptis chinensis extract.Methods In May,2023,the minimum inhibitory concentration(MIC)of Coptis chinensis extract on MDR-Ab was determined by Oxford cup method.The structural damage of MDR-Ab was observed by scanning electron microscope.The effect of Coptis chinensis extract on MDR-Ab biofilm was observed by propidium iodide staining.The extracellular and extracellular adenosine triphosphate(ATP)contents of Coptis chinensis extracts were detected after MDR-Ab treatment.Results The MIC of Coptis chinensis extract on MDR-Ab was 64 mg/mL.The morphology of MDR-Ab treated with Coptis chinensis extract changed,such as depression and distortion,and most of them were dyed red,and the degree of morphological change and the proportion of dyed red were in direct proportion to the concentration of Coptis chinensis extract.The ATP content in MDR-Ab cells decreased gradually with the increase of the concentration of Coptis chinensis extract,and the ATP content outside the cells increased gradually with the increase of the concentration of Coptis chinensis extract.Conclusion Coptis chinensis extract has antibacterial effect on MDR-Ab,and it can exert antibacterial effect by destroying MDR-Ab cell membrane.

关 键 词：黄连 多重耐药鲍曼不动杆菌 生物膜 

分 类 号：R378[医药卫生—病原生物学] R282.71[医药卫生—基础医学]