NapA蛋白诱导巨噬细胞分泌趋化因子单核细胞趋化蛋白1和白细胞介素8的机制  

The mechanism of NapA protein inducing macrophages to secrete chemokine monocyte chemoattractant protein 1 and interleukin 8

作　　者：李杰[1,2] 严洁 杨红霞[4] LI Jie;YAN Jie;YANG Hongxia(Academic Administration Office,Yongzhou Vocational Technical College,Yongzhou 425006,China;Academic Administration office,Xiangtan Medicine Health Vocational College;Department of Neurology,Affiliated Hospital of Yongzhou Vocational Technical College;Medical College of Yongzhou Vocational and Technical College)

机构地区：[1]永州职业技术学院教务处,湖南永州425006 [2]湘潭医卫职业技术学院教务处 [3]永州职业技术学院附属医院神经内科 [4]永州职业技术学院医学院

出　　处：《包头医学院学报》2024年第8期15-20,共6页Journal of Baotou Medical College

基　　金：湖南省教育厅科学研究项目(NO.19C1853);湖南省自然科学基金项目(NO.2022JJ60082)。

摘　　要：目的:研究幽门螺杆菌NapA蛋白诱导巨噬细胞分泌趋化因子单核细胞趋化蛋白1(monocyte chemoattractant protein-1,MCP-1)和白细胞介素8(interleukin-8,IL-8)的机制。方法:利用NapA蛋白处理巨噬细胞,然后使用ELISA法检测上清中MCP-1和IL-8的表达量。使用C29、ST2825、SB203580、SP600125以及PDTC和巨噬细胞预先共孵育1 h,分别抑制Toll样受体2(toll-like receptors 2,TLR2)、髓样分化因子(myeloid differentiation factor 88,MyD88)、核糖核酸酶p蛋白亚基p38(ribonuclease p-protein subunit p38,p38)、应激活化蛋白激酶(c-Jun N-terminal kinase,c-Jun)和核因子κB(nuclear factor kappa B,NF-κB)的活性,然后再加入NapA蛋白孵育4 h,收集上清并检查其中MCP-1和IL-8的表达量。结果:NapA蛋白刺激巨噬细胞后,MCP-1和IL-8的表达量明显高于未处理组。利用抑制剂C29抑制TLR2的活性,NapA蛋白诱导的MCP-1和IL-8表达量降低。使用ST2825、PDTC以及SB203580分别抑制MyD88、NF-κB以及p38的活性,能够降低NapA蛋白诱导巨噬细胞分泌MCP-1和IL-8的能力,但是抑制c-Jun不影响NapA蛋白诱导巨噬细胞分泌MCP-1和IL-8。结论:幽门螺杆菌NapA蛋白通过TLR2/MyD88/NF-κB通路诱导巨噬细胞分泌MCP-1和IL-8。Objective:To investigate the mechanism of H.pylori NapA protein inducing the secretion of monocyte chemoattractant protein-1(MCP-1)and interleukin-8(IL-8)in macrophages.Methods:Macrophages were stimulated by NapA,then chemokines MCP-1 and IL-8 in supernatant were detected through ELISA.Macrophages were pre-incubated with C29,ST2825,SB203580,SP600125 and PDTC for 1 h to inhibit the activity of TLR2,MyD88,p38,c-Jun and NF-κB,respectively.Then NapA protein was added and incubated for 4 h.The supernatant was collected and the expression levels of MCP-1 and IL-8 were examined.Results:The expression levels of MCP-1 and IL-8 in macrophages stimulated by H.pylori NapA protein were significantly higher than those in untreated group.When the activity of TLR2 was inhibited by C29,the expression of MCP-1 and IL-8 induced by NapA protein decreased.Inhibition of MyD88 NF-κB and p38 decreased the ability of macrophages to secrete MCP-1 and IL-8 induced by NapA protein.However,inhibition of c-Jun did not affect the secretion of MCP-1 and IL-8 by macrophages induced by NapA protein.Conclusion:H.pylori NapA protein induces macrophages to secrete chemokines MCP-1 and IL-8 via TLR2/MyD88/NF-κB pathway.

关 键 词：幽门螺杆菌 NapA蛋白 Toll样受体2 趋化因子 单核细胞趋化蛋白1 白细胞介素8 

分 类 号：R392[医药卫生—免疫学]