Th17和调节性T细胞在人类免疫缺陷病毒疾病进展中的作用及其调控机制  

Role and regulatory mechanisms of Th17 and regulatory T cells in the progression of HIV infection

作　　者：李延卿[1] 任伟宏[1] 张岱[1] 李文博[1] 张旭冉 桑锋[1] Li Yan-qing;Ren Wei-hong;Zhang Dai;Li Wen-bo;Zhang Xu-ran;Sang Feng(The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou,Henan 450000,China)

机构地区：[1]河南中医药大学第一附属医院,河南郑州450000

出　　处：《中国现代医学杂志》2024年第16期45-50,共6页China Journal of Modern Medicine

基　　金：河南省科技攻关课题(No:182102310312,No:212102311126);河南省科技研发计划联合基金(No:232301420080);河南省2023年度河南省“双一流”创建学科中医学科学研究专项(No:HSRP-DFCTCM-2023-1-31)。

摘　　要：目的本研究通过观察人类免疫缺陷病毒(HIV)感染者外周血Th17、Treg细胞及其相关转录因子、细胞因子的表达,进一步揭示Th17、Treg细胞的调控机制及其在HIV感染者疾病进展中的作用。方法选取2019年1月—2019年10月河南省上蔡县HIV感染者80例作为研究组,选取同地区健康人群20例作为对照组。采用流式细胞术检测外周血CD4^(+)T、CD8^(+)T及外周血单个核细胞Th17、Treg,酶联免疫吸附试验检测血浆中细胞因子白细胞介素-17(IL-17)、IL-23水平,实时聚合酶链反应检测转录因子ROR-γt及Foxp3 mRNA表达,Pearson法分析Th17、Treg与CD4^(+)T的相关性。结果研究组CD4^(+)T、CD4^(+)T/CD3+T、CD4^(+)T/CD8^(+)T低于对照组(P<0.05),CD8^(+)T和CD8^(+)T/CD3+T高于对照组(P<0.05)。研究组Th17/CD4^(+)T、Th17/Treg低于对照组(P<0.05),Treg/CD4^(+)T高于对照组(P<0.05)。研究组ROR-γtmRNA高于对照组(P<0.05),Foxp3mRNA低于对照组(P<0.05)。研究组IL-17、IL-23水平低于对照组(P<0.05)。Pearson相关性分析结果表示,Th17细胞百分比与CD4^(+)T细胞计数呈正相关(r=0.293,P<0.05),Treg细胞百分比与CD4^(+)T细胞计数呈负相关(r=-0.198,P<0.05)。结论HIV感染能降低Th17细胞、升高Treg细胞,破坏机体免疫平衡,其机制与调控转录因子ROR-γt、Foxp3及细胞因子IL-17、IL-23表达有关,Th17细胞、Treg细胞与HIV感染者疾病进展密切相关。Objective To observe the levels of Th17 and Treg cells along with their associated transcription factors and cytokines in peripheral blood of HIV-infected individuals,and to further reveal the regulatory mechanisms of Th17 and Treg cells and their roles in the progression of HIV infection.Methods From January 2019 to October 2019,the 80 HIV-infected individuals from a region in Henan Province were selected as the study group,and 20 healthy people from the same area were included as the control group.The levels of CD4^(+)and CD8^(+)T cells in peripheral blood and Th17 and Treg cells within peripheral blood mononuclear cells were detected by flow cytometry.The plasma levels of interleukin(IL)-17 and IL-23 were detected by enzyme linked immunosorbent assay.The mRNA expressions of transcription factors ROR-γt and Foxp3 were detected by quantitative real-time polymerase chain reaction.Pearson or Spearman method was used to analyze the correlations between levels of Th17 and Treg cells and the count of CD4^(+)T cells.Results The absolute count of CD4^(+)T cells and ratios of CD4^(+)/CD3+T cells and CD4^(+)/CD8^(+)T cells in the study group were lower than those in the control group(P<0.05),while the absolute count of CD8^(+)T cells and the ratio of CD8^(+)/CD3+T cells in the study group were higher than those in the control group(P<0.05).The ratios of Th17/CD4^(+)T cells and Th17/Treg cells in the study group were lower than those in the control group(P<0.05),whereas the ratio of Treg/CD4^(+)T cells in the study group was higher than that in the control group(P<0.05).Compared with the control group,the mRNA expression of ROR-γt was higher(P<0.05),and that of Foxp3 was lower in the study group(P<0.05).The levels of IL-17 and IL-23 in the study group were lower than those in the control group(P<0.05).Pearson correlation analysis revealed that the percentage of Th17 cells was positively correlated with the count of CD4^(+)T cells(r=0.293,P<0.05),and that the percentage of Treg cells was negatively correlated with the co

关 键 词：获得性免疫缺陷综合征 人类免疫缺陷病毒 辅助性T细胞17 调节性T细胞 ROR-γt FOXP3 

分 类 号：R512.91[医药卫生—内科学]