川芎-独活-牛膝配伍在骨关节炎发病中TH17/Treg细胞免疫平衡的调节作用  

The regulatory effect of Chuanxiong-Duhuo-Achyranthes in the immune balance of TH17/Treg cells in the pathogenesis of osteoarthritis

作　　者：尹超逸 陈芷晴 张斌山 YIN Chaoyi;CHEN Zhiqing;ZHANG Binshan(Dongguan Hospital,Guangzhou University of Chinese Medicine,Dongguan Guangdong 523000,China)

机构地区：[1]广州中医药大学东莞医院,广东东莞523000

出　　处：《四川中医》2024年第7期64-68,共5页Journal of Sichuan of Traditional Chinese Medicine

基　　金：广东省中医药局科研项目(编号:20223019);东莞市科技特派员项目(编号:20221800500472);东莞市2020年中医学术流派传承工作室建设项目。

摘　　要：目的:探讨川芎-独活-牛膝配伍在骨关节炎发病中Th17/Treg细胞免疫平衡的调节作用。方法:购置SPF级无菌C57BL/6小鼠40只,随机取小鼠10只为空白对照组;剩余30只小鼠采用Hulth-Telhag方法构建膝关节炎动物模型,并随机分为模型组、异戊酸组和川芎-独活-牛膝配伍组,各10只。空白对照组和模型组腹腔灌注等体积生理盐水,异戊酸组腹腔灌胃异戊酸,川芎-独活-牛膝配伍组采用川芎-独活-牛膝水煎液灌胃,各组均持续7d干预,比较各组HE染色结果、炎性因子水平及Th17/Treg细胞免疫。结果:HE染色下异戊酸组伴有轻度关节软骨破坏,可见少许炎症细胞浸润;川芎-独活-牛膝配伍组关节软骨破坏不明显,炎症细胞亦相对较少;川芎-独活-牛膝配伍组IL-17、IL-6、IL-4低于异戊酸组(P<0.05);IL-10和TGF高于异戊酸组(P<0.05);流式细胞仪测定结果表明:川芎-独活-牛膝配伍组干预7d后Th17细胞低于异戊酸组和模型组(P<0.05);Treg细胞高于异戊酸组和模型组(P<0.05)。结论:川芎-独活-牛膝配伍用于骨关节炎中能减轻膝关节滑膜及软组织损伤,降低体内炎性因子水平,其机制可能与Th17/Treg细胞免疫平衡的调节有关。Objective:To investigate the regulatory effect of the combination of Chuanxiong-Duhuo-Achyranthes on the immune balance of Th17/Treg cells in the pathogenesis of osteoarthritis.Methods:40SPF grade sterile C57BL/6mice were purchased,and 10mice were randomly selected as blank control group;the remaining 30mice were constructed by Hulth-Telhag method to establish an animal model of knee arthritis,and randomly divided into model group,Isovaleric acid group and Chuanxiong-Duhuo-Achyranthes compatibility group,each with 10animals.The blank control group and the model group were given intraperitoneal perfusion with equal volume of normal saline,the isovaleric acid group was given isovaleric acid by intraperitoneal gavage,and the Chuanxiong-Duhuo-Achyranthes compatibility group was given the Chuanxiong-Duhuo-Achyranthes decoction by intraperitoneal administration.Each group lasted for 7days.After intervention,HE staining results,inflammatory factor levels and Th17/Treg cell immunity were compared in each group.Results:Under HE staining,the isovaleric acid group was accompanied by mild articular cartilage destruction,and a little inflammatory cell infiltration was seen;the articular cartilage destruction was not obvious in the Chuanxiong-Duhuo-Achyranthes combination group,and there were relatively few inflammatory cells;Chuanxiong-Duhuo-Achyranthes IL-17,IL-6and IL-4in the compatibility group were lower than those in the isovaleric acid group(P<0.05);IL-10and TGF were higher than those in the isovaleric acid group(P<0.05);the results of flow cytometry showed that:After 7days of intervention,the Th17cells in the Chuanxiong-Duhuo-Achyranthes compatibility group were lower than those in the isovaleric acid group and the model group(P<0.05);the Treg cells were higher than those in the isovaleric acid group and the model group(P<0.05).Conclusion:The combination of Chuanxiong-Duhuo-Achyranthes for osteoarthritis can reduce the knee joint synovium and soft tissue damage,and reduce the level of inflammatory factors in the body.T

关 键 词：川芎-独活-牛膝配伍 骨关节炎 TH17/TREG细胞 免疫平衡 炎性因子 

分 类 号：R285.5[医药卫生—中药学]