基于TLR4、NF-κB信号通路研究清营汤对LCWE诱导川崎病模型小鼠炎症反应与治疗作用  

Study on the Inflammatory Response and Therapeutic Effect of Qingyingtang on LCWE-induced Kawasaki Disease Model Mouse Based on TLR4 and NF-κB Signaling Pathway

作　　者：颜荆芬 朱焱林 YAN Jingfen;ZHU Yanlin(Shenzhen Luohu District People's Hospital/The Third Affiliated Hospital of Shenzhen University,Shenzhen Guangdong 518000,China)

机构地区：[1]深圳市罗湖区人民医院/深圳大学第三附属医院,广东深圳518000

出　　处：《四川中医》2024年第7期73-78,共6页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:研究清营汤对川崎病模型小鼠的治疗作用。方法:50只符合标准的C57BL/6JSD小鼠随机分成空白对照组(NC)、川崎病模型组(KD)、清营汤低剂量组(QYT-L)、清营汤高剂量组(QYT-H)、阿司匹林组(ASC),每组各10只。通过干酪乳酸杆菌(LCWE)对NC组之外小鼠造模。造模成功后,QYT-L组、QYT-H组、ASC组分别灌胃给予50mg/kg QYT、100mg/kg QYT及50mg/kg ASC,NC组及KD组灌胃等体积PBS,1次/d,持续干预7d。记录小鼠体重变化;心脏超声检查小鼠左心室射血分数(LVEF)与短轴收缩率(FS);酶联免疫吸附测定(ELISA)法测定小鼠血清中IL-6、IL-1β、TNF-α含量;血凝分析仪与ELISA检测血浆纤维蛋白原(FIB)、D-二聚体(D-Dimer)、抗凝血酶Ⅲ(AT-Ⅲ);观察小鼠心脏冠脉组织苏木精-伊红(HE)染色病理学形态变化并通过IPP测定病变范围;蛋白免疫印迹法(WB)检测小鼠心脏冠脉组织NF-κB p65、p-NF-κB p65、TNF-α、TLR4、MyD88蛋白表达情况。结果:治疗后,QYT-L、QYT-H、ASC组小鼠体重、LVEF、FS、血浆FIB水平显著高于KD组(P<0.05);QYT-L、QYT-H组与ASC组干预后的血清TNF-α、IL-6、IL-1β含量、D-Dimer、AT-Ⅲ水平显著低于KD组(P<0.05);KD组冠状动脉壁增厚伴有炎症浸润,QYT-L组略有改善,QYT-H以及ASC组改善明显,IPP测定病变区域面积结果显示,QYT-L、QYT-H、ASC组显著低于KD组(P<0.05);与NC组相比,KD组p-NF-κB p65、TNF-α、TLR4、MyD88蛋白表达量明显增加(P<0.05);与KD组相比,QYT-L、QYT-H、ASC组小鼠p-NF-κB p65、TNF-α、TLR4、MyD88蛋白表达量明显下降(P<0.05)。结论:清营汤能有效改善KD小鼠的心肌功能、凝血功能,抑制TLR4/NF-κB信号通路激活进而抑制炎症因子大量释放,从而发挥对于KD冠脉损伤的治疗作用。Objective:To study the therapeutic effect of Qingyingtang(QYT)on Kawasaki disease(KD)model mouse.Methods:50eligible C57BL/6JSD mice were randomly divided into blank control group(NC),Kawasaki disease model group(KD),QYT low-dose group(QYT-L),QYT high-dose group(QYT-H),and aspirin group(ASC),with 10mice in each group.mouse except for the NC group were modeled by lactobacillus casei cell wall extract(LCWE).After successful modeling,50mg/kg QYT,100mg/kg QYT and 50mg/kg ASC were given by gavage in the QYT-L,QYT-H and ASC groups,respectively,and an equal volume of PBS was given by gavage in the NC group and the KD group,1t/d,for 7d.Changes in body weight of the mice were recorded;cardiac ultrasonography was used to examine the LVEF and FS;enzyme-linked immunosorbent assay(ELISA)to determine the serum levels of IL-6,IL-1β,and TNF-α in mice;hemagglutination analyzer and ELISA to detect plasma fibrinogen(FIB),D-dimer,and antithrombin Ⅲ(AT-Ⅲ);observation of hematoxylin-eosin(HE)staining of coronary arteries of mouse heart tissue for pathomorphic changes and The extent of lesions was determined by IPP;protein westernblotting(WB)was used to detect the expression of NF-κB p65,p-NF-κB p65,TNF-α,TLR4,and MyD88proteins in the coronary tissues of mouse hearts.Results:After treatment,the body weight,LVEF,FS,and plasma FIB levels of mouse in the QYT-L,QYT-H,and ASC groups were significantly higher than those in the KD group(P<0.05);serum TNF-α,IL-6,and IL-1β levels,D-Dimer,and AT-Ⅲ levels were significantly lower than those in the KD group after intervention in the QYT-L,QYT-H,and ASC groups(P<0.05);and KD group Coronary artery wall thickening with inflammatory infiltration was slightly improved in the QYT-L group and significantly improved in the QYT-H and ASC groups,and the results of IPP measurement of lesion area showed that the QYT-L,QYT-H,and ASC groups were significantly lower than that of the KD group(P<0.05);compared with the NC group,the expression of p-NF-κB p65,TNF-α,TLR4,and MyD88proteins was significan

关 键 词：川崎病 冠状动脉炎 清营汤 小鼠 

分 类 号：R285.5[医药卫生—中药学]