女贞子中多种有效化学成分的同步测定方法研究  

Study on the simultaneous analysis of multiple active chemical components in Ligustri Lucidi Fructus

作　　者：金雪琴 漆与白 杨越鹏 李新莹 JIN Xue-qin;QI Yu-bai;Yang Yue-peng;LI Xin-ying(Key Laboratory of Basic Chemistry of the National Ethnic Affairs Commission,School of Chemistry and Environment,Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]西南民族大学化学与环境学院,化学基础国家民委重点实验室,四川成都610041

出　　处：《西南民族大学学报（自然科学版）》2024年第4期399-407,共9页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：四川省自然科学基金项目(2023NSFSC1096)。

摘　　要：建立一种高效液相色谱法同步分析女贞子中咖啡酸、芦丁、槲皮素和熊果酸四种有效化学成分的方法.使用Waters SymmetryshieldTMRP18(4.6×250 mm,5μm)色谱柱,以乙腈-0.1%甲酸水溶液为流动相梯度洗脱,体积流量1.0 mL/min,检测波长范围190~400 nm,柱温30℃,进样量10μL.在此色谱条件下,芦丁、槲皮素、咖啡酸和熊果酸四种成分分离效果良好,在40~250μg/mL、40~400μg/mL、80~700μg/mL和40~400μg/mL范围内各相关系数均大于0.999,不同浓度条件下芦丁、槲皮素、咖啡酸和熊果酸日内精密度试验的平均RSD值分别为0.41%、0.61%、0.61%、0.86%,日间精密度试验的平均RSD值分别为0.43%、0.52%、0.68%、0.83%,稳定性试验的RSD值分别在0.17%~0.67%、0.54%~0.80%、0.32%~0.87%、0.36%~1.29%,平均回收率分别为100.81%、97.68%、102.83%、100.01%.该方法精密度高、稳定性良好,可以对女贞子中芦丁、槲皮素、咖啡酸和熊果酸四种有效成分进行同步准确测定,研究结果可为四种有效化学成分的深入研究以及女贞子的开发应用提供研究基础.In this study,high performance liquid chromatography(HPLC)method was developed for the simultaneous analysis of four active chemical components,namely caffeic acid,rutin,quercetin and ursolic acid in Ligustri Lucidi Fructus.The separa-tion was performed on a Waters Symmetryshield TM RP18(4.6×250 mm,5μm)column with a gradient elution of acetonitrile-0.1%formic acid aqueous solution as the mobile phase,with a volume flow rate of 1.0 mL/min,the detection wavelength in the range of 190~400 nm,the column temperature at 30 degrees Celsius,and the injection volume of 10μL.Under the chromato-graphic conditions,the four active components,namely rutin,quercetin,caffeic acid and ursolic acid,were well separated and simultaneously analyzed,with the correlation coefficients in the ranges of 40~250μg/mL,40~400μg/mL,80~700μg/mL and 40~400μg/mL,respectively,which were all greater than 0.999,and the average RSD values of intra-day precision test for rutin,quercetin,caffeic acid and ursolic acid were 0.41%,0.61%,0.61%,0.86%,and the average RSD values of inter-day precision test were 0.43%,0.52%,0.68%,0.83%,and the RSD values of stability test were in the range of 0.17%~0.67%,0.54%~0.80%,0.32%~0.87%and 0.36%~1.29%,and the average recoveries were 100.81%,97.68%,102.83%and 100.01%,respectively.The method had high precision and good stability,and could be used for the simultaneous and accurate determination of the four active components of Ligustri Lucidi Fructus,namely rutin,quercetin,caffeic acid and ur-solic acid,and the results could provide a research basis for the in-depth study of the four active chemical components as well as the development and application of Ligustri Lucidi Fructus.

关 键 词：咖啡酸 芦丁 槲皮素 熊果酸 高效液相色谱法 

分 类 号：O657.72[理学—分析化学] R284.1[理学—化学]