针刺对自发性高血压大鼠内皮活性因子及相关自主神经递质的影响  

Effects of acupuncture on endothelial active factors and related autonomic neurotransmitters in spontaneous hypertension rats

作　　者：董爱爱 崔依依 王维峰 杨端蓉 武荣林 王文瑞 韩文华 来晓云 王海军 刘海鑫 薛聆 冀来喜 DONG Aiai;CUI Yiyi;WANG Weifeng;YANG Duanrong;WU Ronglin;WANG Wenrui;HAN Wenhua;LAI Xiaoyun;WANG Haijun;LIU Haixin;XUE Ling;JI Laixi(Second Clinical College of Shanxi University of CM,Jinzhong 030619,China;Baoding First Hospital of TCM;Shanxi Provincial Hospital of Acupuncture and Moxibustion;Yuncheng Hospital of TCM)

机构地区：[1]山西中医药大学第二临床学院,晋中030619 [2]保定市第一中医院 [3]山西省针灸医院 [4]运城市中医医院

出　　处：《中国针灸》2024年第8期931-938,965,共9页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金面上项目:81673887;山西省教育厅自然科学类项目:2020L0410;山西省自然科学青年项目:20210302124006;山西中医药大学创新团队项目:2022TD2002;山西中医药大学博士科研启动基金项目:2021BKS05。

摘　　要：目的:观察针刺“降压方”对自发性高血压大鼠(SHR)内皮活性因子及相关自主神经递质的影响,探讨针刺降压的血管调节和中枢调控机制。方法:将30只SPF级雄性SHR随机分为模型组(15只)、针刺组(15只),另以15只京都种Wistar大鼠(WKR)为空白对照组(正常组)。针刺组予“降压方”(双侧“人迎”“曲池”“足三里”“太冲”“内关”)针刺,留针30 min,每日1次,共干预28 d。每周采用激惹评分动态评价大鼠交感激惹表征;通过全自动无创血压测量系统检测大鼠尾动脉血压;ELISA法检测血清降钙素基因相关肽(CGRP)、一氧化氮(NO)、内皮素-1(ET-1)、神经肽Y(NPY)含量;DAB显色原位杂交(CISH)检测颈内动脉、弓状核内皮型一氧化氮合酶(eNOS)及室旁核后部、延髓腹外侧CGRP mRNA表达;液相色谱及质谱联用检测室旁核前部肾上腺素(E)、去甲肾上腺素(NE)含量。结果:与正常组比较,模型组大鼠观察期间各时间点激惹评分及收缩压、舒张压升高(P<0.05);与模型组比较,针刺组大鼠干预第3、4周后激惹评分及干预第2、3、4周后收缩压、舒张压降低(P<0.05)。与正常组比较,模型组大鼠血清CGRP、NO含量降低(P<0.05),血清ET-1、NPY含量及室旁核前部E、NE含量升高(P<0.05);与模型组比较,针刺组大鼠血清CGRP、NO含量升高(P<0.05),血清ET-1、NPY含量及室旁核前部E、NE含量降低(P<0.05)。模型组大鼠颈内动脉中膜增厚且有重构表现,弓状核神经元体积较小;针刺组大鼠颈内动脉各层排布尚可,弓状核小细胞神经元适中。各组大鼠eNOS mRNA在颈内动脉主要表达于各层中平滑肌细胞,而在弓状核主要表达于小细胞神经元。模型组大鼠室旁核后部神经分泌大细胞及小细胞分布较为稀疏,针刺组大鼠两类细胞排布尚可;模型组、针刺组大鼠延髓腹外侧区胶质细胞种类相对较少。各组大鼠CGRP mRNA在室旁核后部主要表达于神经分泌小细胞,Objective To observe the effects of acupuncture at"antihypertensive acupoint prescription"on endothelial active factors and related autonomic neurotransmitters in spontaneous hypertension rats,and explore the vascular regulation and central regulation mechanisms of acupuncture for anti-hypertension.Methods Thirty SPF grade male spontaneous hypertension rats were randomly divided into a model group(15 rats)and an acupuncture group(15 rats).Besides,15 Wistar Kyoto rats were collected as a blank control group(normal group).In the acupuncture group,acupuncture was delivered at the"antihypertensive acupoint prescription"(bilateral"Renying"[ST 9],"Quchi"[LI 11],"Zusanli"[ST 36],"Taichong"[LR 3]and"Neiguan"[PC 6]),with needles retained for 30 min,once daily.The duration of intervention was 28 days.Every week,using the the irritation scale,the sign of sympathetic irritation was evaluated dynamically.The arterial blood pressure of the rats tail was determined,using non-invasive blood pressure measurement system.ELISA was adopted to detect the levels of calcitonin gene-related peptide(CGRP),nitric oxide(NO),endothelin-1(ET-1),neuropeptide Y(NPY)in the serum.DAB chromogenic in situ hybridization(CISH)was provided to detect the mRNA expression of endothelial nitric oxide synthase(eNOS)in the internal carotid artery and the arcuate nucleus(ARC),and that of CGRP in the paraventricular nucleus posterior(PVP)and the ventrolateral medulla(VLM).Liquid chromatography-mass spectrometry(LC-MS)was used to detect the levels of epinephrine(E)and norepinephrine(NE)in the paraventricular nucleus anterior(PVA).Results Compared with the normal group,the irritation scores,systolic blood pressure and diastolic blood pressure were increased at each time point in the model group(P<0.05).When compared with the model group,the irritation scores after the intervention for 3 and 4 weeks,and systolic and diastolic blood pressure after intervention for 2,3 and 4 weeks were reduced in the acupuncture group(P<0.05).In comparison with the normal group,t

关 键 词：自发性高血压 针刺 腧穴降压组方 神经递质 血管内皮活性物质 

分 类 号：R245[医药卫生—针灸推拿学]