2021年江苏省徐州市手足口病和疱疹性咽峡炎病例肠道病毒病原谱分析  

Pathogen spectra of hand,foot and mouth disease and herpangina in Xuzhou,Jiangsu,2021

作　　者：张文婷 杜阳光[1] 高雪 林常青 胡润 童晶[1] Zhang Wenting;Du Yangguang;Gao Xue;Lin Changqing;Hu Run;Tong Jing(Xuzhou Prefectural Center for Disease Control and Prevention,Xuzhou 221006,Jiangsu,China)

机构地区：[1]徐州市疾病预防控制中心,江苏徐州221006

出　　处：《疾病监测》2024年第7期863-868,共6页Disease Surveillance

基　　金：徐州市重点研发计划(社会发展)项目(No.KC22225)。

摘　　要：目的了解江苏省徐州市手足口病(HFMD)和疱疹性咽峡炎(HA)病例肠道病毒病原谱特征,分析柯萨奇病毒A组6型(Cox A6)、柯萨奇病毒A组4型(Cox A4)、柯萨奇病毒A组10型(Cox A10)完整VP1区基因特征。方法用实时荧光定量聚合酶链式反应(PCR)法和巢式PCR法检测HFMD和HA病例样本的肠道病毒血清型;对阳性样本用人横纹肌肉瘤细胞分离病毒,进行VP1区基因测序;基于VP1区全基因序列构建系统进化树,进行同源性分析。结果2021年徐州市引起HFMD的前4位优势肠道病毒是Cox A6、柯萨奇病毒A组16型(Cox A16)、Cox A10和Cox A4;引起HA的是Cox A4、Cox A10、柯萨奇病毒A组2型(Cox A2)和Cox A6。基于VP1区基因构建的系统进化树显示,Cox A6、Cox A10、Cox A4毒株分别为D3a亚型、C2亚型、C2亚型,与中国近年流行株亲缘关系较近;2种疾病分离毒株的VP1区基因无明显差异。结论2021年徐州市HFMD和HA病例的病原谱不完全一致;Cox A10和Cox A4在2种疾病中都属于重要血清型,并和Cox A6、Cox A16共同流行;应持续开展HFMD和HA病原谱变化和病毒基因变异监测,预防疾病暴发。Objective To understand pathogen spectra of hand foot and mouth disease(HFMD)and herpangina in Xuzhou,Jiangsu province,and analyze the VP1 gene characteristics of coxsackievirus A6(Cox A6),coxsackievirus A4(Cox A4),and coxsackievirus A10(Cox A10).Methods Real-time polymerase chain reaction(PCR)and nested PCR amplifications were used to detect the enterovirus serotypes in HFMD and herpangina case samples.Human rhabdomyosarcoma cells were used to isolate the virus strains from positive samples.The gene sequence analysis of the VP1 region were implemented.The phylogenetic tree was constructed according to the entire gene sequence on VP1 region,and the homology analysis was performed.Results In 2021,the top four predominant serotypes of coxsackievirus causing HFMD were Cox A6,Cox A16,Cox A10 and Cox A4,and the top four predominant serotypes of coxsackievirus causing herpangina were Cox A4,Cox A10,Cox A2 and Cox A6 in Xuzhou.The phylogenetic tree constructed based on the VP1 region gene shows that Cox A6,Cox A10,and Cox A4 strains belonged to subtype D3a,C2,and C2,respectively,which had close relationship with reference strains isolated in China in recent years.There was no significant difference in the VP1 region genes between virus strains of two viruses.Conclusion The pathogen spectra of HFMD and herpangina were not completely consistent in Xuzhou.Cox A10 and Cox A4,co-circulating with Cox A6 and Cox A16,were serotypes causing HFMD and herpangina.Continuous surveillance for HFMD and herpangina pathogen spectra and genetic variations should be carried out to prevent outbreaks of HFMD and herpangina.

关 键 词：手足口病 疱疹性咽峡炎 肠道病毒 VP1基因 序列分析 

分 类 号：R211[医药卫生—中医学] R373.2