广州市5892例女性人乳头瘤病毒感染和基因分型特点分析  

Prevalence and genotype distribution of human papilloma virus infection among 5892 female patients in Guangzhou

作　　者：黄演婷[1] 陈小明[1] 曾丽婷 杨小蓉[1,2] 黄兰兰 HUANG Yanting;CHEN Xiaoming;ZENG Liting;YANG Xiaorong;HUANG Lanlan(Laboratory Department of the First Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou,Guangdong 510062,China;不详)

机构地区：[1]广东药科大学附属第一医院检验科,广东广州510062 [2]广东药科大学临床医学院

出　　处：《中国预防医学杂志》2024年第6期746-750,共5页Chinese Preventive Medicine

基　　金：2020年度省级和国家级大学生创新创业训练计划项目(S202010573051)。

摘　　要：目的了解广州市女性人乳头瘤病毒(human papilloma virus,HPV)的感染及基因分型情况,为宫颈癌防治提供流行病学依据。方法采集2021年1—12月广东药科大学附属第一医院门诊及体检的女性宫颈脱落细胞样本,利用聚合酶链式反应(polymerase chain reaction,PCR)-反向点杂交法,分析HPV基因型。结果调查5892名女性,HPV感染820例,感染率13.92%,其中HPV单一基因型感染637例(77.68%),双重感染140例(17.07%),多重感染43例(5.25%)。高危型HPV感染率(9.35%)高于低危型(3.00%)和混合感染型(1.56%)。高危型HPV感染率最高的前5种基因型依次是HPV52(3.60%)、HPV58(1.49%)、HPV16(1.46%)、HPV53(1.17%)和HPV51(0.88%),而低危型HPV感染率最高的前3种基因型依次为HPV44(1.17%)、HPV54(0.70%)和HPV61(0.68%)。各年龄组中,≥60岁组HPV感染率最高(29.34%),不同年龄组之间HPV的感染率差异有统计学意义(χ^(2)=47.44,P<0.05)。结论广州市女性HPV感染率较高,应加强女性预防HPV感染的意识,提高HPV的筛查和疫苗接种率。Objective The current study aimed to investigate the prevalence and genotype distribution of human papilloma virus(HPV)among women in Guangdong province and to provide an epidemiological basis for the prevention and treatment of cervical cancer in this area.Methods Cervical exfoliated cell samples were collected from female outpatients and the health examination center of the First Affiliated Hospital of Guangdong Pharmaceutical University from January 2021 to December 2021.HPV genotyping was performed using Polymerase Chain Reaction(PCR)-reverse dot blot hybridization.Results The overall HPV infection rate was 13.92%(820/5892)in Guangzhou.Among the HPV-positive cases,637(77.68%)were infected with a single HPV genotype,140(17.07%)were infected with a dual HPV genotype and 43(5.25%)with multiple HPV genotypes.The infection rate of high-risk HPV(9.35%)was higher than that of low-risk HPV genotypes(3.00%)and mixed genotypes(1.56%).The top five most prevalent high-risk HPV genotypes were HPV52(3.60%),HPV58(1.49%),HPV16(1.46%),HPV53(1.17%),and HPV51(0.88%),while the top three most prevalent low-risk HPV genotypes were HPV44(1.17%),HPV54(0.70%)and HPV61(0.68%).The highest prevalence of HPV infection was found in women aged≥60 years(29.34%),and significant differences were observed among different age groups(χ^(2)=47.44,P<0.05).Conclusionss The HPV infection rate among women in Guangzhou is relatively high.Efforts should be made to strengthen women's awareness of preventing HPV infection and to improve HPV screening and vaccination coverage.

关 键 词：人乳头瘤病毒 感染率 基因分型 PCR-反向点杂交法 

分 类 号：R512.99[医药卫生—内科学]