OsFK1 encodes C-14 sterol reductase,which is involved in sterol biosynthesis and affects premature aging of leaves in rice  

作　　者：Ping Li Xia Zhang Wuzhong Yin Yang Shui Jie Zhang Nana Xu Dasong Bai Qingxiong Huang Yuanyuan Li Pan Qi Xing Li Qianlong Li Shihong Yang Guotao Yang Hong Chen Peng Qin Yungao Hu Youlin Peng 

机构地区：[1]Rice Research Institute,School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,Sichuan,China [2]Hainan Yazhou Bay Seed Laboratory,Sanya 572025,Hainan,China [3]Rice Research Institute,State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Sichuan Agricultural University,Chengdu 610061,Sichuan,China [4]Yazhouwan National Laboratory,Sanya 572704,Hainan,China

出　　处：《The Crop Journal》2024年第4期1010-1021,共12页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32001491,32360493);Natural Science Foundation of Sichuan Province(2022NSFSC0153,2022NSFSC1754,2023NSFSC1170);the Key Research and Development Program of Sichuan Province(2021YFYZ0016).

摘　　要：The enzyme C-14 sterol reductase is involved in biosynthesis of brassinosteroids(BR)and sterols,as well as plant development.OsFK1,a member of the sterol biosynthesis pathway located in the endoplasmic reticulum(ER),encodes C-14 sterol reductase.However,there is little research on the function of C-14 sterol reductase in rice.Compared with the wild type,an osfk1 mutant showed dwarf phenotype and premature aging in the second leaf during the trefoil stage,and abnormal development of leaf veins during the tillering stage.The osfk1 mutant showed signs of aberrant PCD,as evidenced by TUNEL staining.This suggested that high ROS buildup caused DNA damage and ROS-mediated cell death in the mutant.The osfk1 mutant also showed decreased chlorophyll content and aberrant chloroplast structure.Sequencing of the osfk1 mutant allele revealed a non-synonymous G to A mutation in the final intron,leading to early termination.Here,we identified the OsFK1 allele,cloned it by Mutmap sequencing,and verified it by complementation.HPLC-MS/MS assays demonstrated that the osfk1 mutation caused lower phytosterol levels.These findings showed that the OsFK1 allele encoding C-14 sterol reductase is involved in phytosterol biosynthesis and mediates normal development of rice plants.

关 键 词：Leaf development ROS Phytosterol biosynthesis RICE 

分 类 号：S511[农业科学—作物学]