The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley  

作　　者：Anna Nowicka Martin Kovacik Anna Maksylewicz Przemysław Kopec Ewa Dubas Monika Krzewska Agnieszka Springer Robert E.Hoffie Diaaeldin S.Daghma Zbynek Milec Ales Pecinka Jochen Kumlehn Iwona Zur 

机构地区：[1]The Franciszek Górski Institute of Plant Physiology Polish Academy of Sciences,Niezapominajek 21,30-239 Kraków,Poland [2]Institute of Experimental Botany of the Czech Academy of Sciences,Centre of Plant Structural and Functional Genomics,Šlechtitelu31,77900 Olomouc,Czech Republic [3]Department of Cell Biology and Genetics,Faculty of Science,Palacky UniversityŠlechtitelu°27,77900 Olomouc,Czech Republic [4]Malopolska Centre of Biotechnology,Jagiellonian University,30-387 Kraków,Poland [5]Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Gatersleben,Corrensstraße 3,06466 Stadt Seeland,Germany [6]Institute for Breeding Research on Horticultural Crops,Julius Kühn Institute(JKI)-Federal Research Centre for Cultivated Plants,Erwin-Baur Straße 27,06484 Quedlinburg,Germany [7]Global Change Research Institute Czech Academy of Sciences,Beˇlidla 986/4a,60300 Brno,Czech Republic

出　　处：《The Crop Journal》2024年第4期1064-1080,共17页作物学报（英文版）

基　　金：funded by National Science Center in Poland Grant (2015/18/M/NZ3/00348) to Iwona·Zur;supported by Czech Science Foundation Grant (21-02929S) to Ales Pecinka;European Regional Development Fund project TANGENC (CZ.02.01.01/00/ 22_008/0004581);funded by Ad Agri F (CZ.02.01.01/00/22_008/0004635);supplied by the project “e-Infrastruktura CZ” (e-INFRA CZ LM2018140) supported by the Ministry of Education, Youth and Sports of the Czech Republic

摘　　要：Plant formation from in vitro-cultivated microspores involves a complex network of internal and environmental factors.Haploids/doubled haploids(DHs)derived from in vitro-cultured microspores are widely used in plant breeding and genetic engineering.However,the mechanism underlying the developmental switch from regular pollen maturation towards microspore-derived plant regeneration remains poorly defined.Here,RNA-sequencing was employed to elucidate the transcriptional landscapes of four early stages of microspore embryogenesis(ME)in barley cultivars Golden Promise and Igri,which exhibit contrasting responsiveness to microspore-derived plant formation.Our experiments revealed fundamental regulatory networks,specific groups of genes,and transcription factor(TF)families potentially regulating the developmental switch.We identified a set of candidate genes crucial for genotype-dependent responsiveness/recalcitrance to ME.Our high-resolution temporal transcriptome atlas provides an important resource for future functional studies on the genetic control of microspore developmental transition.

关 键 词：Hordeum vulgare Marker gene Microspore embryogenesis Stress response Transcriptome analysis 

分 类 号：S512.3[农业科学—作物学]