CRISPR/CasRx-mediated resistance to Soybean mosaic virus in soybean  

作　　者：Le Gao Lijun Xie Yanmin Xiao Xinge Cheng Ruosi Pu Ziheng Zhang Yu Liu Shaopei Gao Zilong Zhang Haoran Qu Haijian Zhi Kai Li 

机构地区：[1]MARA Key Laboratory of Biology and Genetics Improvement of Soybean,Zhongshan Biological Breeding Laboratory(ZSBBL),National Innovation Platform for Soybean Breeding and Industry-Education Integration,State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China [2]Department of Horticulture,Beijing Vocational College of Agriculture,Beijing 102442,China [3]College of Agronomy&Biotechnology,China Agricultural University,Beijing 100193,China [4]College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110866,Liaoning,China

出　　处：《The Crop Journal》2024年第4期1093-1101,共9页作物学报（英文版）

基　　金：supported by grants from National Natural Science Foundation of China(32001571);R&D Program of Beijing Municipal Education Commission(KM202212448003,KM202312448004);Science and Technology Innovation Project of Beijing Vocational College of Agriculture(XY-YF-22-02);Zhongshan Biological Breeding Laboratory(ZSBBL-KY2023-03);China Agriculture Research System of MOF and MARA(CARS-04);Jiangsu Collaborative Innovation Center for Modern Crop Production(JCICMCP);Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry(CIC-MCP).

摘　　要：Soybean mosaic virus(SMV),an RNA virus,is the most common and destructive pathogenic virus in soybean fields.The newly developed CRISPR/Cas immune system has provided a novel strategy for improving plant resistance to viruses;hence,this study aimed to engineer SMV resistance in soybean using this system.Specifically,multiple sgRNAs were designed to target positive-and/or negative-sense strands of the SMV HC-Pro gene.Subsequently,the corresponding CRISPR/CasRx vectors were constructed and transformed into soybeans.After inoculation with SMV,39.02%,35.77%,and 18.70%of T_(1)plants were confirmed to be highly resistant(HR),resistant(R),and mildly resistant(MR)to SMV,respectively,whereas only 6.50%were identified as susceptible(S).Additionally,qRT-PCR and DAS-ELISA showed that,both at 15 and 30 d post-inoculation(dpi),SMV accumulation significantly decreased or was even undetectable in HR and R plants,followed by MR and S plants.Additionally,the expression level of the CasRx gene varied in almost all T_(1)plants with different resistance level,both at 15 and 30 dpi.Furthermore,when SMV resistance was evaluated in the T_(2)generation,the results were similar to those recorded for the T_(1)generation.These findings provide new insights into the application of the CRISPR/CasRx system for soybean improvement and offer a promising alternative strategy for breeding for resistance to biotic stress that will contribute to the development of SMV-immune soybean germplasm to accelerate progress towards greater soybean crop productivity.

关 键 词：SOYBEAN RNA virus Soybean mosaic virus RESISTANCE CRISPR/CasRx 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]