Characterization of a 4.1 Mb inversion harboring the stripe rust resistance gene YR86 on wheat chromosome 2AL  

作　　者：Qiang Cao Zhanwang Zhu Dengan Xu Jianhui Wu Xiaowan Xu Yan Dong Yingjie Bian Fugong Ding Dehui Zhao Yang Tu Ling Wu Dejun Han Caixia Lan Xianchun Xia Zhonghu He Yuanfeng Hao 

机构地区：[1]College of Agronomy,Northwest A&F University,Yangling 712100,Shaanxi,China [2]State Key Laboratory of Crop Gene Resources and Breeding,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences(CAAS),Beijing 100081,China [3]Food Crops Institute,Hubei Academy of Agricultural Sciences/Wheat Disease Biology Research Station for Central China,Wuhan 430064,Hubei,China [4]Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610066,Sichuan,China [5]Hubei Hongshan Laboratory,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,Hubei,China [6]CIMMYT-China Office,C/O CAAS,Beijing 100081,China

出　　处：《The Crop Journal》2024年第4期1168-1175,共8页作物学报（英文版）

基　　金：financially supported by the National Key Research and Development Program of China (2022YFD1200900 and 2022YFD1200904);the Agricultural Science and Technology Innovation Program;Fundamental Research Funds for Central NonProfit of Institute of Crop Sciences, CAAS;Shijiazhuang S&T Project (232490022A and 232490432A)

摘　　要：Wheat cultivar Zhongmai 895 was earlier found to carry YR86 in an 11.6 Mb recombination-suppressed region on chromosome 2AL when crossed with Yangmai 16.To fine-map the YR86 locus,we developed two large F2 populations from crosses Emai 580/Zhongmai 895 and Avocet S/Zhongmai 895.Remarkably,both populations exhibited suppressed recombination in the same 2AL region.Collinearity analysis across Chinese Spring,Aikang 58,and 10+wheat genomes revealed a 4.1 Mb chromosomal inversion spanning 708.5-712.6 Mb in the Chinese Spring reference genome.Molecular markers were developed in the breakpoint and were used to assess a wheat cultivar panel,revealing that Chinese Spring,Zhongmai 895,and Jimai 22 shared a common sequence named InvCS,whereas Aikang 58,Yangmai 16,Emai 580,and Avocet S shared the sequence named InvAK58.The inverted configuration explained the suppressed recombination observed in all three bi-parental populations.Normal recombination was observed in a Jimai 22/Zhongmai 895 F2 population,facilitating mapping of YR86 to a genetic interval of 0.15 cM corresponding to 710.27-712.56 Mb falling within the inverted region.Thirty-three high-confidence genes were annotated in the interval using the Chinese Spring reference genome,with six identified as potential candidates for YR86 based on genome and transcriptome analyses.These results will accelerate map-based cloning of YR86 and its deployment in wheat breeding.

关 键 词：Adult-plant resistance Chromosomal inversion Puccinia striiformis Triticum aestivum 

分 类 号：S512.1[农业科学—作物学]