Utilizing auxin dwarf genes to optimize seed yield and lodging resistance in rapeseed  

作　　者：Hongxiang Lou Yan Peng Chunyun Wang Zongkai Wang Bowen Zhao Ali Mahmoud El-Badri Maria Batool Bo Wang Jing Wang Zhenghua Xu Jie Zhao Jie Kuai Guangsheng Zhou 

机构地区：[1]MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River,Hubei Hongshan Laboratory,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,Hubei,China [2]National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,Hubei,China [3]Field Crops Research Institute,Agricultural Research Center,Giza 12619,Giza Governorate,Egypt

出　　处：《The Crop Journal》2024年第4期1208-1221,共14页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China (2021YFD1901200)

摘　　要：Direct-seeding rapeseed production at high plant density raises the risk of lodging.We investigated the use of dwarf genes to improve rapeseed plant architecture to balance yield and lodging.Three genotypes with different plant architectures(dwarf sca^(HS5),semi-dwarf+/sca^(HS5),and tall ^(HS5))were evaluated under varying nitrogen rates(N1,N2,and N3:120,240,and 360 kg N ha^(-1))and plant densities(D1,D2,and D3:15,45,and 75 plants m^(-2))from 2019 to 2022.The results showed that increasing N rate positively influenced yield while decreasing lodging resistance in all genotypes.Increasing plant density(D2-D3)enhanced lodging resistance and yield in sca^(HS5) and+/sca^(HS5),but reduced yield in ^(HS5).Compared to the two parents,+/sca^(HS5) exhibited moderate expressions of IAA3,GH3.15,and SAUR30 in stems under N2D3,resulting in reduced plant height and increased compactness.Additionally,+/sca^(HS5) had a thicker silique layer than ^(HS5) by 14.7%,and it had a significant correlation between branch height/angle and yield.Increasing N rate led to increased lignin and pectin contents,while cellulose content decreased.Increasing plant density resulted in greater stem cellulose content and CSLA3/7 expression in sca^(HS5) and+/sca^(HS5),but decreased in ^(HS5).Compared to ^(HS5),+/sca^(HS5) exhibited higher expressions of ARAD1 and GAUT4,along with a 51.1%increase in pectin content,leading to improved lodging resistance under N2D3.Consequently,+/sca^(HS5) showed a 46.4%higher yield and 38.9%lodging resistance than ^(HS5) under N2D3,while sca^(HS5) demonstrated strong lodging resistance but lower yield potential.Overall,this study underscores the potential of utilizing auxin dwarf genes to optimize the trade-off between yield and lodging resistance in rapeseed and the possibility of maximizing yield potential by optimizing the plant architecture of+/sca^(HS5) through nitrogen reduction and dense planting.

关 键 词：RAPESEED Plant density NITROGEN LODGING AUXIN 

分 类 号：S565.4[农业科学—作物学]