Introgression of lac6/tl1/du13 improves the palatability of japonica rice  

作　　者：Yong Yang Tao Zhang Yuliang Shi Yan Lu Qianfeng Li Xiaolei Fan Lichun Huang Lu Chen Xuetang Song Qing Liu Changquan Zhang Qiaoquan Liu 

机构地区：[1]Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education,Agricultural College of Yangzhou University,Yangzhou 225009,Jiangsu,China [2]Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology,Yangzhou University,Yangzhou 225009,Jiangsu,China [3]Jiang Su(Wu Jin)Rice Research Institute,Changzhou 213175,Jiangsu,China

出　　处：《The Crop Journal》2024年第4期1259-1265,共7页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2023YFD1200901);the National Natural Science Foundation of China(32230074 and 32161143004);the Research Programs from Jiangsu Government(JBGS[2021]001,BZ2021017,and Qing Lan Project);the Foreign Expert Project(G2023014064L);Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX21_3239);the Yangzhou University High-end Talent Program.

摘　　要：Amylose content(AC)is a crucial determinant of the eating and cooking quality(ECQ)of rice,with low AC varieties exhibiting a softer texture and greater stickiness-attributes that enhance palatability and are desirable in specific culinary contexts.To harness these traits,significant efforts have been made to manipulate AC to improve rice ECQ.Our research utilized the MutMap+approach to identify LAC6/TL1,a gene that is an allele of Du13,responsible for low AC.LAC6 encodes a C2H2 zinc finger protein,which specifically increases the splicing efficiency of the Wxb allele without affecting the Wxa allele.Functional studies of LAC6 revealed that its proper integration could rectify the undesirable AC phenotype,whereas mutations within this gene led to reduced AC and were associated with shorter grain length and decreased thousand-grain weight.Despite these drawbacks,such mutations positively impact rice palatability,presenting a trade-off between grain size and eating quality.To address the challenges posed by the reduced grain weight associated with LAC6 mutations,we developed a specific molecular marker for LAC6,which has been effectively used in breeding programs to select lac6/tl1/du13 homozygous individuals with larger grain size.Our findings demonstrate that the“small grain”trait associated with lac6/tl1/du13 can be effectively mitigated through combined phenotype-based and marker-assisted selection.This study highlights the potential of lac6/tl1/du13 as a valuable gene for breeding novel,high-quality soft rice varieties through targeted breeding strategies.

关 键 词：Rice Dull endosperm Amylose content WX BREEDING 

分 类 号：S511[农业科学—作物学]