基于高通量测序的西北太平洋柔鱼食性初步分析  

Preliminary analysis of the feeding habits of Ommastrephes bartramii in the high sea of Northwest Pacific Ocean based on high-throughput sequencing

作　　者：王欣 刘必林[1,2,3,4] 何骏杰 宋林玮 WANG Xin;LIU Bilin;HE Junjie;SONG Linwei(College of Marine Living Resource Sciences and Management,Shanghai Ocean University,Shanghai 201306,China;National Engineering Research Center for Oceanic Fisheries,Shanghai 201306,China;Key Laboratory of SustainableExploitation of Oceanic Fisheries Resources,Ministry of Education,Shanghai 201306,China;Key Laboratory of Sustainable Utilization of Oceanic Fisheries,Ministry of Agriculture and Rural Affairs,Shanghai 201306,China)

机构地区：[1]上海海洋大学海洋生物资源与管理学院,上海201306 [2]国家远洋渔业工程技术研究中心,上海201306 [3]大洋渔业资源可持续开发教育部重点实验室,上海201306 [4]农业农村部大洋渔业可持续利用重点实验室,上海201306

出　　处：《上海海洋大学学报》2024年第4期900-910,共11页Journal of Shanghai Ocean University

基　　金：农业农村部全球渔业资源调查监测评估(公海渔业资源综合科学调查)专项(D-8025-23-1002);上海市高校特聘教授“东方学者”岗位跟踪计划(GZ2022011)。

摘　　要：为深入了解柔鱼食性,探明其在食物网中的位置和生态功能价值,根据2022年6—8月于西北太平洋公海采集的15尾柔鱼样本,采用高通量测序技术分析其食物组成。结果显示,15尾样品的高通量序列为646980条,通过聚类分析共得到624个运算分类单元(OTU),经筛选比对检测到的饵料共计65种,分属6门11纲22目37科51属。研究表明,柔鱼主要摄食头足类、鱼类以及多种浮游动物(包括磷虾类、介形类、水母类等)。优势饵料包括拟沙丁鱼、日本爪乌贼、北方拟黵乌贼、萤乌贼、尾棘背灯鱼等。柔鱼摄食范围广,饵料物种与海区的天然饵料有关;不同胴长组柔鱼表现出不同的摄食偏好。通过柔鱼胃含物的全组成分析,对深度挖掘摄食与柔鱼生长、洄游、繁殖等生活史的关系有重要作用,同时为基于生态系统角度的柔鱼资源可持续开发和养护提供科学依据。In order to understand the feeding habits of Ommastrephes bartramii and explore its position in the food web and ecological function,we analyzed the food composition of 15 Ommastrephes bartramii samples which were collected in the high sea of Northwest Pacific Ocean using high-throughput sequencing.The results showed that there were 646 980 high-quality sequences in 15 samples,a total of 624 operational taxonomic units(OTUs) were obtained through cluster analysis.A total of 65 food species,belonging to 6phyla,11 classes,22 orders,37 families and 51 genera,were detected by screening and comparison.Ommastrephes bartramii fed mainly on cephalopods,fishes,and a variety of zooplanktons(including Euphausiid,Ostracod,Medusae,etc.).The dominant prey species included Sardinops sagax,Onychoteuthis borealijaponica,Gonatopsis borealis,Watasenia scintillans,Notoscopelus caudispinosus,etc.In conclusion,the feeding range of Ommastrephes bartramii was wide,and the food species were related to the natural preys in the sea area.Furthermore,Ommastrephes bartramii showed different feeding preferences among different mantle length groups.The analysis of the whole composition of stomach contents of Ommastrephes bartramii plays an important role in digging out the relationship between feeding and life history,such as growth,migration and reproduction.This provides a scientific basis for the sustainable development and conservation of Ommastrephes bartramii resources from the perspective of ecosystem.

关 键 词：柔鱼 食性 高通量测序 西北太平洋 胃含物 

分 类 号：S931.1[农业科学—渔业资源]