大豆主要饲用性状的遗传分析与QTL定位  

Genetic Analysis and Quantitative Trait Locus Mapping of Major Feeding Traits in Soybean

作　　者：王传之 崔卫东 任海龙 夏明阳 曾仕晓 张建国[1] WANG Chuanzhi;CUI Weidong;REN Hailong;XIA Mingyang;ZENG Shixiao;ZHANG Jianguo(South Pratacultural Center,South China Agricultural University,Guangzhou 510642,China;College of Biology and Food Engineering,Suzhou University,Suzhou 234000,China;Crop Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Crops Genetics and Improvement,Guangzhou 510640,China;Guangzhou Qianyun BIO co.,LTD,Guangzhou 510620,China)

机构地区：[1]华南农业大学南方草业中心,广东广州510642 [2]宿州学院生物与食品工程学院,安徽宿州236065 [3]广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室,广东广州510640 [4]广州千云生物有限公司,广东广州510620

出　　处：《中国草地学报》2024年第8期29-36,共8页Chinese Journal of Grassland

基　　金：国家重点研发计划(2017YFD0101404);国家大豆产业技术体系资金(CARS04);广东省现代农业产业技术体系南方现代草牧业(羊)创新团队(2019KJ127)。

摘　　要：为培育高饲用价值的大豆品种,本研究以饲用性状差异较大的粤夏106和矮生泥豆为亲本,其衍生的F_(2)代为作图群体,利用覆盖大豆全基因组的660对SSR引物,对大豆株高、生物产量、酸性洗涤木质素和相对饲用价值等8个重要饲用性状进行QTL定位。结果表明:大豆株高、主茎节数、主茎分枝数、生物产量和中性洗涤纤维含量、酸性洗涤纤维含量、酸性洗涤木质素含量、相对饲用价值之间均具有显著的相关性(P<0.05)。共检测到18个QTLs,分布在A1、B2、C1、C2、J和L共计6条连锁群上。其中,株高2个、主茎分枝1个、主茎节数3个、生物产量2个,中性洗涤纤维含量3个、酸性洗涤纤维含量1个、酸性洗涤木质素含量3个和相对饲用价值3个,单个QTL可解释的表型贡献率介于2.11%~59.77%,qADF-l、qADL-a1、qADL-c2、qBY-j、qNBMS-l、qNDF-j和qNNMS-l等7个位点为表型贡献率大于15%的主效QTLs。To develop new soybean varieties with high feed value,Yuexia 106 and Aishengnidou,along with their F_(2)population,were used as the mapping population.A total of 660 SSR markers,covering the entire soybean genome,were employed to map quantitative trait locus(QTLs)for eight key feeding traits of soybean,such as plant height,biological yield,acid detergent lignin(ADL),relative feeding value(RFV),and so on.The results showed that significant phenotypic correlations(P<0.05)were observed among biological yield,branch number on the main stem,number of nodes per main stem,plant height,neutral detergent fiber(NDF),acid detergent fiber(ADF),ADL,and RFV.A total of 18 QTLs were detected across six linkage groups(A1,B2,C1,C2,J,and L).These included 2 QTLs for plant height,1 for nodes per main stem,3 for branch number on the main stem,2 for biological yield,3 for NDF content,1 for ADF content,3 for lignin content,and 3 for RFV.The phenotypic variance explained by individual QTL ranged from 2.11%to 59.77%,with qADF-l,qADL-a1,qADL-c2,qBY-j,qNBMS-l,qNDF-j,and qNNMS-l having phenotypic contributions greater than 15%.

关 键 词：全株大豆 QTL 生物产量 相对饲用价值 

分 类 号：S548[农业科学—作物学]