Large-field objective lens for multi-wavelength microscopy at mesoscale and submicron resolution  

作　　者：Xin Xu Qin Luo Jixiang Wang Yahui Song Hong Ye Xin Zhang Yi He Minxuan Sun Ruobing Zhang Guohua Shi 

机构地区：[1]School of Biomedical Engineering(Suzhou),Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230026,China [2]Jiangsu Key Laboratory of Medical Optics,Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Science,Suzhou 215163,China

出　　处：《Opto-Electronic Advances》2024年第6期41-56,共16页光电进展（英文）

基　　金：supported by National Key R&D Program of China(grant no.2022YFC2404201);the Chinese Academy of Sciences Project for Young Scientists in Basic Research(grant no.YSBR067).

摘　　要：Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view,typically around 1 mm.This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample,such as different brain areas.In addition,conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo.Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm,a numerical aperture of 0.5,and a working wavelength range from 400 to 1000 nm.We achieved a resolution of 0.74μm in fluorescent beads imaging.The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system,a confocal laser scanning system,and a two-photon imaging system.This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.

关 键 词：mesoscopic objective lens large field-of-view high resolution MULTI-WAVELENGTH wide-field microscopy confocal laser scanning microscopy 

分 类 号：TH742[机械工程—光学工程]