木质素基多酚的制备及生物活性  

Preparation and Bioactivities of Lignin-based Polyphenols

作　　者：李龙 卫海琳[3] 王雯蕾 张评浒 周永红 杨晓慧[1,2] LI Long;WEI Hailin;WANG Wenlei;ZHANG Pinghu;ZHOU Yonghong;YANG Xiaohui(Institute of Chemical Industry of Forest Products,CAF,Key Lab.of Biomass Energy and Material,Jiangsu Province,Key Lab.of Chemical Engineering of Forest Products,National Forestry and Grassland Administration,National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass,Nanjing 210042,China;Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing Forestry University,Nanjing 210037,China;Medical College,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]中国林业科学研究院林产化学工业研究所、江苏省生物质能源与材料重点实验室、国家林业和草原局林产化学工程重点实验室、林木生物质低碳高效利用国家工程研究中心,江苏南京210042 [2]南京林业大学江苏省林业资源高效加工利用协同创新中心,江苏南京210037 [3]扬州大学医学院,江苏扬州225009

出　　处：《林产化学与工业》2024年第4期121-129,共9页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(32071718);江苏省生物质能源与材料重点实验室资助项目(JSBEM-S-201803)。

摘　　要：针对木质素活性位点低,活性差的问题,利用酸性熔盐水合物通过一锅法脱甲基化及酚化改性制备了木质素基多酚化合物即连苯三酚改性木质素(PEHL)。实验结果表明:脱甲基化木质素(DEHL)和PEHL的反应位点显著增加,含酚羟基的量由原料酶解木质素(EHL)中的1.25 mmol/g分别提高到3.49和5.70 mmol/g。DPPH·清除实验发现,木质素基多酚化合物的抗氧化活性显著增加,与EHL对DPPH·抑制率(25.4%)相比,质量浓度10 mg/L下DEHL和PEHL的DPPH·清除率分别提高了46.9和64.4个百分点;PEHL展示了良好的抑菌活性,添加量1 g/L时对大肠杆菌和金色葡萄球菌的抑制率分别为85.22%和99.76%;木质素基多酚还表现出较低的细胞毒性。Aiming at the problems of low active sites and poor activity of lignin,pyrogallol modified enzymatic hydrolyzed lignin(PEHL),a lignin-based polyphenol compound,was prepared by one-pot demethylation and phenolic modification from acidic molten salt hydrate.The experimental results demonstrated a significant increase in the reaction sites of demethylated enzymatic hydrolyzed lignin(DEHL)and PEHL,with phenolic hydroxyl groups increasing from 1.25 mmol/g in raw enzymatic hydrolyzed lignin(EHL)to 3.49 and 5.70 mmol/g,respectively.Furthermore,DPPH·scavenging experiments revealed a significant enhancement in the antioxidant activities of lignin-based polyphenol compounds,and the DPPH·scavenging rates of DEHL and PEHL increased by 46.9%and 64.4%,respectively.Moreover,PEHL showed promising antibacterial activity,and low cytotoxicity with inhibition rates of E.coli and S.aureus by 85.22%and 99.76%,respectively.

关 键 词：木质素 结构改性 脱甲基化木质素 酚化改性 生物活性 

分 类 号：TQ35[化学工程]