基于多元统计分析方法研究地黄在慢性不可预知温和应激抑郁模型大鼠的入血成分及其代谢产物  

Components and metabolites into blood of Rehmannia glutinosa in chronic unpredictable mild stress depression model rats based on multivariate statistical analysis

作　　者：苗金歌 田萍 韩德恩[1] 马开[2] 刘一菲 史思佳 李红伟[1] 潘晓乐 刘改枝[1] MIAO Jinge;TIAN Ping;HAN Deen;MA Kai;LIU Yifei;SHI Sijia;LI Hongwei;PAN Xiaole;LIU Gaizhi(School of Pharmacy,Henan University of Chinese Medicine,Henan Zhengzhou 450046,China;Institute of Chinese Medicine,Henan Integrative Medicine Hospital/Henan Academy of Chinese Medicine,Henan Zhengzhou 450004,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南省中西医结合医院河南省中医药研究院中药所,河南郑州450004

出　　处：《中国医院药学杂志》2024年第15期1759-1765,共7页Chinese Journal of Hospital Pharmacy

基　　金：河南省科技攻关项目(编号:232102311214);河南省中医药科学研究专项重大课题(编号:20-21ZYZD13);河南省中医药科学研究专项课题(编号:2022ZY1150);河南省中医药研究院基本业务费课题(编号:2004823、2204977)。

摘　　要：目的:研究地黄灌胃给予慢性不可预知温和应激(chronic unpredictable mild stress,CUMS)模型大鼠入血成分及其代谢成分。方法:采用CUMS加孤养建立SD大鼠抑郁症模型,运用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UPLCQ-Orbitrap HRMS)技术结合多元统计分析方法,分析模型组、地黄7.2 g·kg^(–1)组的血浆样本,寻找地黄抗抑郁的潜在药效成分。结果:从血浆样品正交偏二乘判别分析(OPLS-DA)VIP>1和T检验P<0.05的差异药源性成分中,共推断出了53个血中移行成分,其中24个原形成分、29个代谢产物(其原形成分分别为梓醇、桃叶珊瑚苷、京尼平苷、毛蕊花糖苷、地黄苦苷、地黄苦苷元)。结论:通过对地黄在CUMS大鼠体内入血成分和代谢产物的鉴定,为地黄抗抑郁药效物质基础及作用机制研究提供了数据支撑。OBJECTIVE To explore the components and metabolites into blood of Rehmannia glutinosa in a rat model of chronic unpredictable mild stress(CUMS).METHODS The depression model of Sprague-Dawley(SD)rats was established by CUMS and solitary rearing.The plasma samples of model and dosing groups were detected by ultra-high performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS).And potential antidepressant components of Rehmannia glutinosa were examined by multivariate statistical analysis.RESULTS 53 transitional components in blood were inferred from the difference between model and Rehmannia glutinosa dosing groups in VIP value of OPLS-DA>1 and P<0.05 in T test.There were 24 prototypic components and 29 metabolites(original components were catalpol,aucubin,geniposide,verbascoside,rehmapicroside&rehmapicrogenin).CONCLUSION Through the identification of the components and metabolites into blood of Rehmannia glutinosa in CUMS model rats,it provides data supports for researches on material basis and mechanism of antidepressant effect of R.glutinosa.

关 键 词：地黄 超高效液相色谱-四极杆/静电场轨道阱高分辨质谱 多元统计分析 入血成分 血清药物化学 

分 类 号：R284.1[医药卫生—中药学] R969.1[医药卫生—中医学]