禾谷炭疽菌Velvet蛋白CgrVosA的生物学功能  

Biological function of a Velvet protein CgrVosA in Colletotrichum graminicola

作　　者：王利亚 张莹 周双针 唐雯 李晓宇[1] 柳志强[1] WANG Liya;ZHANG Ying;ZHOU Shuangzhen;TANG Wen;LI Xiaoyu;LIU Zhiqiang(School of Life and Health Sciences,Hainan University,Haikou 570228,Hainan,China)

机构地区：[1]海南大学生命健康学院,海南海口570228

出　　处：《微生物学通报》2024年第8期2922-2933,共12页Microbiology China

基　　金：国家自然科学基金(32160041)。

摘　　要：【背景】禾谷炭疽菌(Colletotrichum graminicola)是侵染玉米的主要病原菌之一,每年造成严重的经济损失,直接威胁全球粮食安全。Velvet蛋白是真菌特有的一种保守性功能蛋白,它们在调控真菌生长及发育过程中发挥着重要作用。【目的】从禾谷炭疽菌中鉴定Velvet蛋白CgrVosA,并分析其生物学功能。【方法】利用同源重组的方法构建禾谷炭疽菌CgrvosA敲除突变株和互补菌株,通过测定其营养生长、胁迫因子响应、分生孢子产量及萌发、致病性等明确其功能。【结果】CgrvosA编码一个含有425个氨基酸的蛋白,有一个典型的Velvet蛋白结构域。与野生型菌株相比,敲除突变株ΔCgrvosA生长速度减慢,并且对NaCl和H_(2)O_(2)敏感;ΔCgrvosA卵圆形分生孢子的产量和萌发率显著降低,并且形态明显大于野生型菌株。【结论】禾谷炭疽菌Velvet蛋白CgrVosA参与调控菌株营养生长、对高渗和氧化应激的响应、卵圆形分生孢子的产生和萌发等过程。[Background]Colletotrichum graminicola,one of the main pathogens infecting maize,causes serious economic losses and directly threatens the global food security every year.Velvet proteins are fungal unique proteins with conserved functions,playing a role in regulating the growth and development of fungi.[Objective]To study the biological functions of a Velvet protein CgrVosA identified from C.graminicola.[Methods]The CgrvosA-knockout mutant and complementary strain were constructed by homologous recombination.The vegetative growth,stress response,conidial yield,germination,and pathogenicity of the strains were determined and compared to reveal the functions of CgrVosA.[Results]CgrvosA encoded a 425-residue protein with a typical Velvet protein domain.Compared with the wild type,ΔCgrvosA exhibited slow growth,high sensitivity to NaCl and H_(2)O_(2),enlarged oval conidia,and reduced yield and germination rate of oval conidia.[Conclusion]The Velvet protein CgrVosA is involved in the regulation of vegetative growth,response to hyperosmotic and oxidative stress,and oval conidial production and germination of C.graminicola.

关 键 词：禾谷炭疽菌 Velvet蛋白 生物学功能 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治]