羊源粪肠球菌多位点序列分型及其耐药特性和毒力基因检测分析  

Multilocus sequence typing,antibiotic resistance,and virulence genes of Enterococcus faecalis from sheep

作　　者：邓亚飞 吴佳颖 张留君 闫康 辛洪雷 贺绍君[1,2] DENG Yafei;WU Jiaying;ZHANG Liujun;YAN Kang;XIN Honglei;HE Shaojun(College of Animal Science,Anhui Science and Technology University,Fengyang 233100,Anhui,China;Anhui Province Key Laboratory of Animal Nutrition Regulation and Health,Fengyang 233100,Anhui,China;Zhongyuan Animal Husbandry Development Center of Linquan County,Linquan 236400,Anhui,China)

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100 [2]动物营养调控与健康安徽省重点实验室,安徽凤阳233100 [3]临泉县中原牧业发展中心,安徽临泉236400

出　　处：《微生物学通报》2024年第8期3070-3084,共15页Microbiology China

基　　金：安徽省教育厅自然科学研究重大项目(2023AH040282);安徽省现代农业产业技术体系建设专项资金([2022]10);乡村振兴专项项目(2021XCZX05)。

摘　　要：【背景】粪肠球菌(Enterococcus faecalis)作为一种普遍存在的条件致病菌,能引起严重感染,并能作为高耐药/毒力因子的储存和传播者对公共安全造成严重威胁。【目的】掌握安徽地区羊源粪肠球菌ST型分布特征及耐药、毒力特性,并分析其耐药基因、毒力基因与ST型之间的相关性,评估其潜在致病性及对公共卫生的危害性。【方法】以2021−2023年采集自安徽地区的64份羊直肠棉拭子为样本对粪肠球菌进行分离鉴定,采用多位点序列分型(multilocus sequence typing,MLST)方法对菌株进行分型鉴定,Kirby-Bauer(K-B)纸片扩散法测定粪肠球菌对16种抗菌药物的敏感性,并检测9种耐药基因及14种毒力基因的分布。【结果】共分离得到61株粪肠球菌,鉴定为24种ST型,其中9种为新发现ST型;奈替米星(72.13%)、丁胺卡那(75.41%)、四环素(55.74%)和红霉素(63.93%)的耐药比例最高,少数对万古霉素(9.84%)、利奈唑胺(11.48%)耐药,多重耐药(multi-drug resistance,MDR)率为57.38%;耐药基因ermB(77.05%)、aac(6')/aph(2')(52.46%)、tem(55.74%)的携带率最高,vanC(8.20%)、mefA(1.64%)的携带率最低;毒力基因cpd(100%)、efaA(98.36%)、ebp(95.08%)和fsr(81.97%)的携带率最高,hyl(11.48%)的携带率最低;部分耐药基因、毒力基因的分布在不同ST型之间具有显著差异(P<0.05)。【结论】羊源粪便粪肠球菌中携带高耐药和高水平毒力基因,且耐药特性及毒力的分布与ST型有着较高的相关性。[Background]As a common opportunistic pathogen,Enterococcus faecalis can cause severe infections and pose a serious threat to public safety as a carrier of high resistance/virulence factors.[Objective]To master the sequence type(ST)distribution,antibiotic resistance,and virulence of sheep-derived E.faecalis strains in Anhui Province,analyze the correlations of antibiotic resistance and virulence genes with ST,and evaluate the potential threat to the public health.[Methods]E.faecalis was isolated from 64 sheep anal swab samples collected from Anhui during 2021−2023.Multilocus sequence typing(MLST)was employed to classify and identify the isolates.Kirby Bauer(K-B)test was carried out to determine the sensitivity of E.faecalis isolates to 16 antibiotics.Furthermore,the distribution of 9 resistance genes and 14 virulence genes was tested.[Results]A total of 61 strains of E.faecalis were isolated and identified as 24 STs,of which 9 were newly discovered STs.Most of the strains showed resistance to netilmicin(72.13%),amikacin(75.41%),tetracycline(55.74%),and erythromycin(63.93%).A few strains were resistant to vancomycin(9.84%)and linezolid(11.48%).with And the multi-drug resistance(MDR)rate was 57.38%.The carrier rates of resistance genes ermB(77.05%),aac(6')/aph(2')(52.46%),and tem(55.74%)were high,while those of vanC and mefA were low at 8.20%and 1.64%,respectively.The carrying rates of virulence genes cpd(100%),efaA(98.36%),ebp(95.08%),and fsr(81.97%)were high,while it was the lowest for hyl(11.48%).The distribution of some resistance and virulence genes showed differences among different STs(P<0.05).[Conclusion]Enterococcus faecalis from sheep feces carry high resistance and high virulence genes,and the distribution of resistance and virulence is highly correlated with STs.

关 键 词：粪肠球菌 多位点序列分型 耐药特性 毒力基因 

分 类 号：S852.61[农业科学—基础兽医学]