青藏高原东缘溪流型小微湿地沉积物古菌群落的海拔分布格局及驱动因素  

Elevational distribution pattern and driving factors of archaeal communities in the sediments of small streams along the eastern Tibetan Plateau

作　　者：郭云 仲波[2] 李娜英 李贤祥 张慧 杨早 蒋海波[2] 王晓锋 杨刚[1] 何奕忻[2] Guo Yun;Zhong Bo;Li Naying;Li Xianxiang;Zhang Hui;Yang Zao;Jiang Haibo;Wang Xiaofeng;Yang Gang;He Yixin(School of Life Sciences and Engineering,Southwest University of Science and Technology,Mianyang 621010,P.R.China;Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,P.R.China;School of Life Sciences,Sichuan University,Chengdu 610065,P.R.China;College of Geography and Tourism,Chongqing Normal University,Chongqing 400047,P.R.China)

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621010 [2]中国科学院成都生物研究所,成都610041 [3]四川大学生命科学学院,成都610065 [4]重庆师范大学地理与旅游学院,重庆400047

出　　处：《湖泊科学》2024年第5期1506-1515,I0021-I0026,共16页Journal of Lake Sciences

基　　金：国家自然科学基金项目(32271676,42077038);中国博士后科学基金项目(2023M733439)联合资助。

摘　　要：小微湿地广泛分布于河流源头区域,对维持生态系统稳定性以及提供生态系统服务发挥着重要作用,微生物在其中扮演着重要角色,但当前对小微湿地沉积物中微生物群落尤其是古菌群落的海拔分布格局尚不清楚。因此,基于16S rRNA基因测序技术,本研究调查了岷江上游5个海拔梯度(1000、1600、2000、2700和3200 m)溪流型小微湿地的水文特征、水体理化性质、沉积物古菌群落的组成和多样性等指标。结果表明,岷江上游溪流型小微湿地沉积物中古菌群落组成在海拔梯度上存在显著差异,群落α多样性随海拔升高而显著增加,β多样性在海拔梯度上差异显著。距离衰减分析表明,古菌群落的时空分布与海拔梯度显著相关,进一步的冗余分析证实了溶解性总氮、总氮、溶解性总磷及pH是影响古菌群落结构的主要水体理化因素,浊度和水深是影响古菌群落结构的主要水文因素。综上所述,本研究厘清了岷江上游溪流型小微湿地沉积物古菌群落沿海拔梯度的分布格局,揭示了溪流型小微湿地中水文和水体理化性质共同调控沉积物古菌群落的海拔分布格局,为理解青藏高原自然小微湿地古菌群落海拔分布的生物地理分布提供了科学依据。Small and micro wetlands including small streams are widely distributed in the source areas of rivers,which play an important role in maintaining the ecosystem stability and providing ecosystem services.However,the distribution of microbial communities,especially the archaeal communities,in the stream sediments along different elevations is still unclear.To supplement the theoretical framework within this field,we conducted a study in five stream type small micro wetlands of the upper Minjiang River along different altitudinal gradients(1000,1600,2000,2700 and 3200 m)by using 16S rRNA illumina sequencing technology and monitoring the physicochemical properties of the water body as well as hydrological parameters.The alpha diversity of the archaeal community increased significantly with elevation,and the beta diversity varied significantly across the elevation gradient.Habitat difference was the key factor driving the altitudinal distribution pattern of archaeal communities in the stream type small micro wetlands sediments of the upper Minjiang River.Redundancy analysis confirmed that dissolved total nitrogen,total nitrogen,dissolved total phosphorus and pH were the main physical and chemical factors affecting the archaeal community structure,and turbidity and water depth were the main hydrological factors affecting the archaeal community structure.In conclusion,this study showed that hydrological factors and water body physicochemical properties jointly regulate the altitudinal distribution pattern of stream type small micro wetlands archaeal communities.This study provides a scientific basis for understanding the biogeographic distribution of the altitudinal distribution of natural small micro wetland archaeal communities in the Tibetan Plateau.

关 键 词：古菌 群落组成 多样性 水体理化性质 水文 

分 类 号：Q938[生物学—微生物学]