1株新型鹅星状病毒的全基因序列分析  

Whole Gene Sequence Analysis of a Novel Goose Astrovirus

作　　者：赵瑞宏[1,2] 胡晓苗 侯宏艳[1,2] 周学利 潘孝成[1,2] 戴银 ZHAO Rui-hong;HU Xiao-miao;HOU Hong-yan(Institute of Animal Husbandry and Veterinary Science,Anhui Academy of Agricultural Science/Livestock and Poultry Epidemic Diseases Research Center of Anhui Province,Hefei,Anhui 230031;Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering,Hefei,Anhui 230031)

机构地区：[1]安徽省农业科学院畜牧兽医研究所/安徽省畜禽疫病研究中心,安徽合肥230031 [2]畜禽产品安全工程安徽省重点实验室,安徽合肥230031

出　　处：《安徽农业科学》2024年第16期87-90,共4页Journal of Anhui Agricultural Sciences

基　　金：安徽省科技重大专项(202003a06020012);安徽省家禽产业技术体系项目(AHCYJSTX-06)。

摘　　要：为研究新型鹅星状病毒(Novel goose astroviruses,N-GAstV)的遗传进化特征,获得了鹅星状病毒安徽分离株AstV/AHHX的全长基因,为7251 nt。测序分析显示:AstV/AHHX具有典型的新型鹅星状病毒基因组特征。AstV/AHHX与分离株GDZJ37以及山东分离株G538和G576的遗传距离较近;同时与毒株GDZJ37、G538和G576的ORF2氨基酸序列同源性达100%,ORF1a和ORF1b的同源性也有99.6%以上,推测它们由同一毒株演化而来。ORF2氨基酸序列位点分析显示有12高变异位点,分别是第36(Y/S/H)、60(V/I)、224(T/A)、228(A/S/T)、229(P/Q)、289(T/A)、376(T/A)、456(D/E)、540(Q/L)、608(S/T)、610(E/D/G)、614(N/A/T)位氨基酸。其中第224、228、229、608、614位的氨基酸突变可能导致病毒蛋白质的构象及功能发生变化,进而改变病毒的致病能力。In order to study the genetic and evolutionary characteristics of a novel goose astrovirus(N⁃GAstV),the full⁃length genes of Anhui strain AstV/AHHX were cloned in this study,and it was 7251 nt.Sequencing analysis showed that AstV/AHHX had typical characteristics of N⁃GAstV.The phylogenetic tree showed that AstV/AHHX was relatively close to GDZJ37 isolates from China,G538 and G576 isolates from Shandong Province in recent years.The homology of ORF2 with GDZJ37,G538 and G576 was 100%,and the homology of ORF1a and ORF1b was more than 99.6%.Presumably they evolved from the same strain.Amino acid sequence analysis of ORF2 revealed 12 highly variable sites.They were the amino acid sites,36(Y/S/H),60(V/I),224(T/A),228(A/S/T),229(P/Q),289(T/A),376(T/A),456(D/E),540(Q/L),608(S/T),610(E/D/G),614(N/A/T).Amino acid mutations at position 224,228,229,608 and 614 may lead to changes in the confor⁃mation and function of viral proteins,then the pathogenic ability of the virus was altered.

关 键 词：新型鹅星状病毒 全基因 序列分析 组织分布 

分 类 号：S852.65[农业科学—基础兽医学]