天目地黄4个变异株形态观测及倍性鉴定  

Morphological Observation and Ploidy Identification of 4 Mutant Strains of Rehmannia chingii

作　　者：宋利娜 张华丽 衣彩洁 赵世伟 冯慧 弓传伟 孙丽萍 SONG Li-na;ZHANG Hua-li;YI Cai-jie(Bejing Key Laboratory of Greening Plants Breeding/Beijing Academy of Forestry and Landscape Architecture,Beijing 100102)

机构地区：[1]绿化植物育种北京市重点实验室/北京市园林绿化科学研究院,北京100102

出　　处：《安徽农业科学》2024年第16期107-110,共4页Journal of Anhui Agricultural Sciences

基　　金：北京市公园管理中心科技课题(ZX2021)。

摘　　要：在连续开展3年多的地黄属植物收集选育工作过程中,发现了4株表观形态有所变化的不同变异株,编号分别为T-1、T-2、T-3、T-4,对其形态指标进行测量统计及数据分析后发现,与天目地黄相比形态差异明显。具体差异表现为:T-1主茎明显短缩;T-2柱头外露于花瓣,叶片长宽比变小;T-3柱头外漏于花瓣,主茎明显短缩;T-4单叶叶面积增大近3倍,主茎明显短缩近无。为明确变异株染色体倍性,用流式细胞仪进行倍性检测分析,结果显示T-1、T-2、T-3峰值均与原株一致,鉴定为2倍体,T-4峰值约为原株的2倍,鉴定为4倍体。In the process of collecting and breeding Rehmannia for more than three years,four single plants with apparent morphological varia⁃tion were found,numbered as T⁃1,T⁃2,T⁃3 and T⁃4,respectively.The plant morphological indexes were measured and the results showed that the differences of morphological indexes were obvious compared with the original species.The main stem of T⁃1 was obviously shortened.The stigma of T⁃2 was exposed to the petals and the length⁃to⁃width ratio of the leaves was smaller.The stigma of T⁃3 was exposed to the petals and the main stem was obviously shortened.The single leaf area of T⁃4 was increased by nearly 3 times and the main stem was significantly short⁃ened.In order to clarify the chromosome ploidy of the mutant plants,the flow cytometry was used for ploidy identification.The results showed that T⁃1,T⁃2 and T⁃3 were identified as diploid,but T⁃4 was identified as tetraploid.

关 键 词：天目地黄 地黄属 变异单株 柱头外露 倍性检测 

分 类 号：Q944[生物学—植物学]