正交试验法优化小麦胚芽中总黄酮的提取条件  

Extraction Conditions of Total Flavonoids from Wheat Germ Optimized by Orthogonal Method

作　　者：李保利[1] 吕会超[1] 殷美丽[1] 郑永军[1] 陈怡如 LI Bao-li;LV Hui-chao;YIN Mei-li;ZHENG Yong-jun;CHEN Yi-ru(Anyang Institute of Technology,Henan Anyang 455000,China)

机构地区：[1]安阳工学院,河南安阳455000

出　　处：《广州化工》2024年第15期54-58,共5页GuangZhou Chemical Industry

基　　金：河南省亚临界高效萃取重点实验室;河南省科技厅项目:艾草精油、艾草粕活性成分分离及应用(232102310353)。

摘　　要：小麦胚芽是小麦中最珍贵的部分,富含黄酮类化合物、谷胱甘肽以及小麦胚芽凝聚素等多种有益物质。在我们的生活中,小麦胚芽被利用得还不够充分。因此,研究采用乙醇回流法对麦胚中总黄酮进行了提取。采用紫外分光光度法,使用显色剂,建立芦丁标准曲线。通过开展单因素和正交试验优化,最终确定最佳条件:料液比为1∶40 g/mL,乙醇体积分数为70%,提取时间为2 h,提取温度为70℃,总黄酮的提取率为0.325 0%。Wheat germ is the most valuable part of wheat and is rich in many beneficial substances,such as flavonoids,glutathione and wheat germ coagulant.Nowadays,wheat germ is not more fully utilized except as animal feed in our life.Therefore,total flavonoids in wheat germ were extracted by the test using ethanol reflux method.A rutin standard curve was constructed by UV spectrophotometric method using chromogenic agent.The extraction criteria of total flavonoids in wheat germ were optimized by single-factor experiment and orthogonal experiment.Finally,the best extraction condition was obtained:the material-liquid ratio was 1∶40 g/mL,the volume fraction of ethanol was 70%,the extraction time was 2 h,and the extraction temperature was 70 ℃.The extraction rate of total flavonoids could reach 0.325 0% under this extraction condition.

关 键 词：小麦胚芽 总黄酮 乙醇回流 

分 类 号：O658.9[理学—分析化学]