胶原蛋白水解物缓解去势小鼠骨质疏松作用机理的探究  

Exploration of the Mechanism of Collagen Hydrolysate in Alleviating Osteoporosis in Castrated Mice

作　　者：杜博玮 李承明[1] Du Bowei;Li Chengming(Beijing University of Chemical Technology,Beijing 100029)

机构地区：[1]北京化工大学,北京100029

出　　处：《明胶科学与技术》2024年第1期31-41,共11页The Science and Technology of Gelatin

摘　　要：胶原蛋白水解物被认为拥有促进成骨和成血管以及起到防止骨质疏松的作用。我们通过生物信息学和建立去势小鼠骨质疏松症模型探究了胶原水解物(CH)对骨质疏松症有益作用的潜在机制。本研究发现,CH可以使24个与骨质疏松和成血管相关基因差异表达,差异基因的富集分析确定了20个KEGG通路。通过对筛选的十个通路进行分析,并结合STITCH数据库的探究,发现了Kdr、Erbb2、Wnt3a、Jagl、Fltl为CH调控的中枢基因。特别是,MAPK信号通路被确定为共享通路中的潜在关键通路。这个研究结果表明,胶原蛋白水解物可以通过MAPK信号通路促进成血管并抑制骨质疏松。Collagen hydrolysate is thought to have the ability to promote osteogenesis and angiogenesis,as well as play a role in preventing osteoporosis.We explored the underlying mechanisms of the beneficial effects of collagen hydrolysate(CH)on oste-oporosis through bioinformatics and established of an castrated mouse model of osteoporosis.In this study,it was found that CH could differentially express 24 genes associated with osteoporosis and angiogenesis,and 20 KEGG pathways were identified by enrichment analysis of differential genes.Through the analysis of the ten pathways screened and the exploration of the STITCH database,Kdr,Erbb2,Wnt3a,Jagl and Fltl were found to be the central genes regulated by CH.In particular,the MAPK signaling pathway was identified as a potentially critical pathway in the shared pathway.The results of this study suggest that collagen hydrolysate can promote angiogenesis and inhibit osteoporosis through the MAPK signaling pathway.

关 键 词：胶原水解物 骨质疏松 成血管 差异基因 信号通路 中枢基因 

分 类 号：R68[医药卫生—骨科学]