Exploiting viral vectors to deliver genome editing reagents in plants  

作　　者：Yilin Shen Tao Ye Zihan Li Torotwa Herman Kimutai Hao Song Xiaoou Dong Jianmin Wan 

机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization,Jiangsu Collaborative Innovation Centre for Modern Crop Production,Jiangsu Engineering Research Center for Plant Genome Editing,Nanjing Agricultural University,Nanjing 210095,China [2]Zhongshan Biological Breeding Laboratory,Nanjing 210014,China [3]Hainan Seed Industry Laboratory,Sanya 572025,China [4]State Key Laboratory of Crop Gene Resources and Breeding,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《aBIOTECH》2024年第2期247-261,共15页生物技术通报（英文版）

基　　金：supported by STI 2030–Major Projects(2023ZD04074);the Natural Science Foundation of Jiangsu Province(BK20210384 and BK20212010);the Agricultural Science and Technology Innovation Program of Jiangsu Province[CX(22)3153];project of the Zhongshan Biological Breeding Laboratory(BM2022008-02);the Hainan Seed Industry Laboratory(B21HJ1004);the Guidance Foundation of the Sanya Institute of Nanjing Agricultural University(NAUSY-ZZ01);the Jiangsu Specially Appointed Professor Program.

摘　　要：Genome editing holds great promise for the molecular breeding of plants,yet its application is hindered by the shortage of simple and effective means of delivering genome editing reagents into plants.Conventional plant transformation-based methods for delivery of genome editing reagents into plants often involve prolonged tissue culture,a labor-intensive and technically challenging process for many elite crop cultivars.In this review,we describe various virus-based methods that have been employed to deliver genome editing reagents,including components of the CRISPR/Cas machinery and donor DNA for precision editing in plants.We update the progress in these methods with recent successful examples of genome editing achieved through virus-based delivery in different plant species,highlight the advantages and limitations of these delivery approaches,and discuss the remaining challenges.

关 键 词：Plant genome engineering Genome editing CRISPR/Cas Virus-based delivery 

分 类 号：Q943.2[生物学—植物学]