PLIN1基因InDel位点检测及其与同羊尾脂和生长性状的关联分析  

Detection of InDel loci of PLIN1 gene and its association analysis with tail fat and growth traits in Tong sheep

作　　者：邱彦博 阮子豪 易晓华 谢浦航 袁婷婷 孙秀柱[2] QIU Yanbo;RUAN Zihao;YI Xiaohua;XIE Puhang;YUAN Tingting;SUN Xiuzhu(College of Animal Science and Technology,Northwest Agriculture and Forestry University,Yangling 712100,China;College of Grassland and Grassland,Northwest Agriculture and Forestry University,Yangling 712100,China)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]西北农林科技大学草业与草原学院,陕西杨凌712100

出　　处：《黑龙江畜牧兽医》2024年第15期43-48,118,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家牧草产业技术体系项目(CARS-34);畜禽种质资源精准鉴定项目“绵羊高繁性状精准评价和分子鉴定”(202121)。

摘　　要：为了探讨同羊PLIN1基因结构及PLIN1基因的插入/缺失(inserted/deletion,InDel)位点对同羊尾脂和体尺性状的影响,试验对PLIN1基因结构进行了生物信息学分析,并测量了166只健康同羊的尾脂和体尺性状;通过测量同羊表型数据、提取基因组DNA,并采用PCR扩增、琼脂糖凝胶电泳和Sanger测序等方法检测PLIN1基因的InDel位点和分型情况,然后对PLIN1基因的InDel位点与同羊尾脂和体尺性状进行关联分析。结果表明:PLIN1基因位于绵羊的18号染色体上,有8个外显子和7个内含子。绵羊、山羊、普通牛、欧亚野猪、智人、小鼠的PLIN1蛋白结构高度保守,原鸡和绿头鸭的PLIN1蛋白结构与其他物种存在差异,以上8个物种的PLIN1蛋白有10个共同基序,2个特定的保守结构域,PLIN1基因在物种进化中相对保守。同羊的PLIN1基因存在1个26 bp(CGATCCTCGGTGCCCCAGAAACATTC)的InDel位点,包含DD、II和ID 3种基因型,I和D等位基因频率分别为0.2018和0.7982,II、ID和DD基因型频率分别为0.0663,0.2711,0.6627,该位点处于中间多态性(0.250.05);该位点对公羊的体尺性状无显著影响(P>0.05);但该位点的II基因型母羊背高显著高于ID和DD基因型(P<0.05)。说明PLIN1基因的InDel位点可被作为母羊育种的有用分子标记。To investigate the effects of structure and inserted/deletion(InDel)variant of PLIN1 gene on tail fat and growth traits of Tong sheep,bioinformatics of the structure of PLIN1 gene was analyzed,and tail fat and growth traits from 166 healthy Tong sheep were measured.The InDels loci and typing of PLIN1 gene were detected by Tong sheep phenotypic data measurement,genomic DNA extraction,PCR,agarose gel electrophoresis and Sanger sequencing,and then the polymorphisms of PLIN1 gene were analyzed by correlating them with the tail fat and body size traits of Tong sheep.The results showed that the PLIN1 gene was located on chromosome 18 of sheep,consisting of 8 exons and 7 introns.The protein structure of PLIN1 across Ovis aries,Capra hircus,Bos taurus,Sus scrofa,Homo sapiens,and Mus musculus was highly conserved.The PLIN1 protein structure of Gallus gallus and Anas platyrhynchos was different from that of other species.Across these eight species,PLIN1 protein of the above 8 species exhibits 10 conserved motifs and 2 specific conservative domains,indicating that PLIN1 gene was relatively conserved in species evolution.The PLIN1 gene in Tong sheep had an InDel site of 26 bp(CGATCCTCGGTGCCCCAGAAACATTC),including DD,II,and ID genotypes.The allele frequencies of the I and D were 0.2018 and 0.7982,respectively,and the frequencies of the II,ID,and DD genotypes were 0.0663,0.2711,and 0.6627,respectively.This locus was in the intermediate polymorphism(0.250.05);the locus had no significant effect on the growth traits of rams(P>0.05);however,the dorsal height of ewes of II genotype at this locus was significantly higher than that of ID and DD genotypes(P<0.05).The results indicated that the Indel locus of PLIN1 gene could be used as a useful molecular marker for ewe breeding.

关 键 词：脂滴包被蛋白1(PLIN1) 同羊 尾脂 插入/缺失(InDel) 生长性状 关联分析 

分 类 号：S826.8[农业科学—畜牧学] Q78[农业科学—畜牧兽医]