添加不同比例全株玉米青贮对合作猪小肠组织结构和肠道屏障功能相关基因表达的影响  

Effects of adding different proportions of whole corn silage on the expression of genes related to intestinal tissue structure and intestinal barrier function in Hezuo pigs

作　　者：刘祥年 殷熙彤 唐于然 王鹏飞[1] 闫尊强 滚双宝[1,2] LIU Xiangnian;YIN Xitong;TANG Yuran;WANG Pengfei;YAN Zunqiang;GUN Shuangbao(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Gansu Modern Pig Engineering Technology Research Center,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070 [2]甘肃省现代养猪工程技术研究中心,兰州730070

出　　处：《黑龙江畜牧兽医》2024年第15期84-92,共9页Heilongjiang Animal Science And veterinary Medicine

基　　金：甘肃省教育科技创新专项(GSSYLXM-02);现代丝路寒旱农业科技支撑项目(GSLK-2022-10);甘肃省科技重大专项(22ZD6NA044)。

摘　　要：为了探究日粮中添加不同比例全株玉米青贮对合作猪小肠组织结构和肠道屏障功能相关基因的影响,试验将32头健康状况良好、体重相近的断奶合作猪仔猪随机分为4组,每组8头(公母各半),对照组饲喂基础日粮,5%组、10%组和15%组分别饲喂添加5%、10%和15%全株玉米青贮日粮,120 d后,每组随机选取3头合作猪屠宰,取小肠各段组织样本,一部分用4%多聚甲醛溶液固定,苏木精-伊红(H.E.)染色后测定小肠绒毛高度和隐窝深度,计算绒毛高度/隐窝深度;另一部分采用实时荧光定量PCR检测小肠肠道屏障功能相关基因mRNA相对表达量。结果表明:与对照组相比,10%和15%组小肠各段绒毛高度均增加,隐窝深度均变浅,绒毛高度/隐窝深度均增大,其中10%组小肠各段绒毛高度均显著升高(P<0.05),十二指肠的隐窝深度显著变浅(P<0.05),十二指肠和空肠的绒毛高度/隐窝深度显著增大(P<0.05);5%组、10%组和15%组的物理屏障相关基因[闭锁小带蛋白-1(ZO-1)、闭合蛋白(Occludin)、连接黏附分子A(JAM-A)、封闭蛋白-1(Claudin-1)]、化学屏障相关基因[β防御素1(PBD1)、分泌型黏蛋白2(MUC2)、肠三叶因子(TFF3)]、抗炎因子[白细胞介素-10(IL-10)和转化生长因子-β1(TGF-β1)]mRNA相对表达量均增加,促炎因子基因[Toll样受体2(TLR2)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)]mRNA相对表达量均降低;且10%组小肠各段的ZO-1、Claudin-1、MUC2、IL-10 mRNA相对表达量均显著升高(P<0.05);10%组十二指肠和空肠中JAM-A、TFF3、TGF-β1 mRNA相对表达量均显著升高(P<0.05),IL-6 mRNA相对表达量显著降低(P<0.05)。说明添加10%全株玉米青贮的日粮可更好地改善合作猪肠组织结构,增强肠道屏障功能,促进肠道健康。In order to investigate the effects of adding different proportions of whole corn silage to diets on genes related to small intestinal tissue structure and intestinal barrier function in Hezuo pigs,thirty-two healthy weaned Hezuo piglets with similar body weights were randomly divided into four groups with eight piglets in each(half male and half female).The pigs in the control group were fed a basal diet,and pigs in the 5%,10%and 15%groups were fed diets with the addition of 5%,10%and 15%of whole corn silage diets,respectively.After 120 d of feeding,three Hezuo pigs were randomly selected from each group and slaughtered.Tissue samples were taken from various segments of the small intestine,and one part was fixed with 4%paraformaldehyde and stained with hematoxylin-eosin(H.E.)to determine the height of the small intestinal villi and the depth of the crypts,and then the villus height/crypt depth was calculated;and the other part was used for the detection of the relative expression of mRNA of genes related to the small intestinal intestinal barrier function by real-time fluorescence quantitative PCR.The results showed that compared with the control group,the villus height of all segments of the small intestine in the 10%and 15%groups increased;the crypt depth became shallower,and the villus height/crypt depth increased,of which the villus height of all segments of the intestine in the 10%group was significantly higher than that in the control group(P<0.05);the crypt depth of the duodenum became significantly shallower(P<0.05),and the villus height/crypt depth of the duodenum and the jejunum was significantly higher(P<0.05);in the 5%,10%,15%group,the relative mRNA expression of physical barrier-related genes(zoster occludin-1[ZO-1],Occludin,junctional adhesion molecule A[JAM-A],Claudin-1),chemical barrier-related genes(β-defensin 1[PBD1],Secretory mucin 2[MUC2],intestinal trichome factor[TFF3]),and anti-inflammatory factors(interleukin-10[IL-10]and transforming growth factor-β1[TGF-β1])were increased,and the exp

关 键 词：合作猪 全株玉米青贮 肠组织结构 肠道屏障 基因表达 

分 类 号：S816.5[农业科学—饲料科学] Q78[农业科学—畜牧学]