托曲珠利混悬液对人工感染仔猪球虫病的临床预防效果试验  

The experiment of clinical preventive effects of totricolil suspension on artificial infection with piglet coccidiosis

作　　者：李文慧 张岩 王丽 蔡明烩 孙永学[1] LI Wenhui;ZHANG Yan;WANG Li;CAI Minghui;SUN Yongxue(College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;Qingdao Continent Animal Pharmaceutical Co.,Ltd.,Qingdao 266109,China)

机构地区：[1]华南农业大学兽医学院,广州510642 [2]青岛康地恩动物药业有限公司,山东青岛266109

出　　处：《黑龙江畜牧兽医》2024年第15期99-103,120,121,共7页Heilongjiang Animal Science And veterinary Medicine

摘　　要：为了研究托曲珠利混悬液对仔猪等孢球虫感染的预防效果,试验对猪源球虫卵囊进行分离纯化及扩繁,建立了人工感染仔猪球虫病模型,通过测定仔猪增重、腹泻情况及克粪便球虫卵囊数(OPG)确定最佳感染剂量,然后将90头3日龄仔猪随机分为感染对照组、参比品组和受试品组,每组30头,仔猪6日龄时对参比品组和受试品组分别灌服5%托曲珠利混悬液受试品和参比品,24 h后3组分别口服最佳感染剂量的孢子化卵囊,测定各组仔猪球虫卵囊阳性率与感染量,计算粪便性状评分、腹泻率、料重比、死亡率、肠道黏膜病变得分等。结果表明:最佳感染剂量为1×10^(4)个孢子化卵囊。受试品组与参比品组粪便中球虫卵囊阳性率在第3~11天极显著低于感染对照组(P<0.01),受试品组与参比品组之间差异不显著(P>0.05),受试品组与参比品组粪便中球虫克卵囊减少率均达100%。受试品组与参比品组第7,14天腹泻率和粪便性状评分差异不显著(P>0.05),均显著低于感染对照组(P<0.05)。受试品组与参比品组仔猪8~14天平均日增重差异不显著(P>0.05),均显著高于感染对照组(P<0.05)。受试品组与参比品组料重比均低于同时期的感染对照组,受试品组死亡率为3.3%,参比品组死亡率为0。受试品组和参比品组小肠黏膜无明显病变,接种卵囊后第7,14天黏膜病变评分差异不显著(P>0.05),均显著低于感染对照组(P<0.05)。说明5%托曲珠利混悬液受试品可有效预防猪等孢球虫病,其临床效果与市售5%托曲珠利混悬液参比品相当。In order to study the preventive effects of toltrazuril suspension on Cystoisospora suis infection in piglets, in the test, coccidia oocysts were isolated and purified and expanded to establish a model of artificial infection of piglets with coccidiosis;the optimal infection dose was determined by measuring the weight gain, diarrhea, and the number of fecal coccidia oocysts(OPG) per gram of piglets. 90 piglets at 3 days of age were randomly divided into infection control group, reference group and test group, with 30 piglets in each group. At 6 days of age, piglets were dosed with 5% toltrazuril suspension in the reference group and the test group respectively, and 24 h later, the three groups were orally administered the optimal infective dose of sporulated oocysts respectively. The positive rate of coccidia oocysts and the amount of infection in each group were determined, and the fecal trait score and diarrhea rate, feed-to-weight ratio and mortality rate, and intestinal mucosal lesions were calculated. The results showed that the optimal infective dose was 1×10^(4) sporulated oocysts. The positive rate of coccidia oocysts in the feces of the test group and the reference group was significantly lower than that of the infection control group from the 3rd to the 11th day(P<0.01), and the difference between the test group and the reference group was not significant(P>0.05). The reduction rate of coccidia oocysts per gram in the feces of both the test group and the reference group reached 100%. The diarrhea rate and fecal trait score on day 7, 14 were significantly lower in the test and reference groups than in the infection control group(P<0.05), and the difference between the test and reference groups was not significant(P>0.05). The average daily weight gain of piglets in the test group and the reference group was significantly higher than that of the infection control group from 8 to 14 days(P<0.05);the difference between the test group and the reference group was not significant(P>0.05). The feed-to-weight r

关 键 词：猪等孢球虫 人工感染 托曲珠利 临床验证试验 腹泻 

分 类 号：S855.9[农业科学—临床兽医学]