支气管肺泡灌洗液及血清NF-κB、TLR-2、TLR-4、TNF-α在肺炎诊断中的应用价值  

The application value of bronchoalveolar lavage fluid and serum NF-κB,TLR-2,TLR-4,TNF-αin the diagnosis of pneumonia

作　　者：金美玉 王冠 林文婷 吴艳峰[2] JIN Meiyu;WANG Guan;LIN Wenting;WU Yanfeng(Jilin University Hospital,Changchun 130012,China;The Second Hospital of Jilin University,Changchun 130022,China)

机构地区：[1]吉林大学校医院,吉林长春130012 [2]吉林大学第二医院,吉林长春130022

出　　处：《中国实验诊断学》2024年第8期915-918,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨支气管肺泡灌洗液(BALF)及血清核转录因子-κB(NF-κB)、Toll样受体2(TLR-2)、Toll样受体4(TLR-4)、肿瘤坏死因子α(TNF-α)在肺炎诊断中的应用价值。方法选取2023年1月—2024年3月于吉林大学第二医院就诊的57例肺炎患者纳入研究组,另取同期体检的健康者57例纳入对照组,均采集外周静脉血与肺泡灌洗液,检测NF-κB、TLR-2、TLR-4、TNF-α水平,采用Pearson相关性分析患者血清及BALF中NF-κB、TLR-2、TLR-4、TNF-α的相关性。结果研究组患者血清NF-κB(41.74±4.75)μg/L、TLR-2(5.32±1.12)ng/L、TLR-4(6.44±1.61)ng/L、TNF-α(4.36±1.03)ng/L水平高于对照组,差异有统计学意义(P<0.05)。研究组患者BALF中NF-κB(8.19±2.62)μg/L、TLR-2(2.97±0.79)ng/L、TLR-4(4.24±1.18)ng/L、TNF-α(2.04±0.59)ng/L水平高于对照组,差异有统计学意义(P<0.05)。Pearson相关性分析显示,患者血清及BALF中NF-κB、TLR-2、TLR-4、TNF-α水平呈正相关性(P<0.05)。结论肺炎患者血清及BALF中NF-κB、TLR-2、TLR-4、TNF-α均呈高表达,联合检测有助于早期诊断及评估病情。Objective To explore the diagnostic value of bronchoalveolar lavage fluid(BALF)and serum nuclear transcription factor kappa B(NF kappa B),Toll like receptor 2(TLR-2),Toll like receptor 4(TLR-4),and tumor necrosis factor alpha(TNF alpha)in pneumonia.Methods 57 pneumonia patients who visited our hospital from January 2023 to March 2024 were selected as the study group,and 57 healthy individuals who underwent physical examinations at the hospital during the same period were selected as the control group.Peripheral venous blood and alveolar lavage fluid were collected to detect the levels of NF-κB,TLR-2,TLR-4,and TNF-α.Pearson correlation analysis was used to analyze the correlation between NF-κB,TLR-2,TLR-4,and TNF-αin the patient's serum and BALF.Results The serum levels of NF-κB(41.74±4.75)μg/L,TLR-2(5.32±1.12)ng/L,TLR-4(6.44±1.61)ng/L,and TNF-α(4.36±1.03)ng/L in the study group were higher than those in the control group,and the differences were statistically significant(P<0.05).The levels of NF-κB(8.19±2.62)μg/L,TLR-2(2.97±0.79)ng/L,TLR-4(4.24±1.18)ng/L,and TNF-α(2.04±0.59)ng/L in BALF of patients in the study group were higher than those in the control group,and the differences were statistically significant(P<0.05).Pearson correlation analysis showed that the levels of NF-κB,TLR-2,TLR-4,and TNF-αin the patient's serum and BALF were positively correlated(P<0.05).Conclusion NF-κB,TLR-2,TLR-4,and TNF-αare highly expressed in the serum and BALF of pneumonia patients.Combined detection is helpful for early diagnosis and evaluation of the condition.

关 键 词：肺炎 支气管肺泡灌洗液 血清 NF-ΚB TLR-2 TLR-4 TNF-Α 

分 类 号：R563.1[医药卫生—呼吸系统]