黄毛耳草中东莨菪内酯的HPLC含量测定及提取工艺优化研究  

HPLC Content Determination and Extraction Process Optimization of Scopolamine in Herba Hedyotids Chrysotridhae

作　　者：金贤武 周燕霞 JIN Xian-wu;ZHOU Yan-xia(Drug Testing Branch o f Shangrao Inspection,Testing and Certification Institute,Shangrao 334000,China;School o f Pharmacy,Jiangxi Medical College,Shangrao 334000,China)

机构地区：[1]上饶市检验检测认证院药品检测分院,江西上饶334000 [2]江西医学高等专科学校药学院,江西上饶334000

出　　处：《宜春学院学报》2024年第6期17-20,125,共5页Journal of Yichun University

基　　金：上饶市社会发展领域科技计划医疗卫生指导性项目(编号:2023CZDX31)。

摘　　要：目的:建立黄毛耳草中东莨菪内酯的HPLC含量测定方法,并优化其提取工艺。方法:色谱柱waters symmetry C_(18)(4.6 mm×250 mm,5μm),流动相乙腈0.1%磷酸水,检测波长345 nm,流速0.8 mL/min,进样量10μL,测定东莨菪内酯含量。采用超声提取法,以乙醇浓度、超声时间、超声功率和料液比为考察因素,以东莨菪内酯含量为评价指标,优化其提取工艺。结果:最佳提取工艺为乙醇浓度74%、提取时间45 min、超声功率250 W、液料比25∶1。精密称取3份黄毛耳草样品,每份2.0 g,此条件下东莨菪内酯的提取含量为为81.97μg/g,与预测值81.41μg/g无显著性差异。结论:本试验建立的HPLC法可用于黄毛耳草中东莨菪内酯的含量测定,所优化的提取工艺稳定可靠、简便可行。Objective:To establish an HPLC method for the determination of scopolamine in Herba Hedyotids Chrysotridhae and optimize its extraction process.Method:The content of scopoletin was determined using Waters Symmetry C_(18)(4.6 mm)×250 mm,5μm Chromatographic column,acetonitrile 0.1%phosphoric acid water as mobile phase,detection wavelength of 345 nm,flow rate of 0.8 mL/min,injection volume of 10μL.Using the ultrasonic extraction method,based on a single factor experiment using ethanol concentration(A),ultrasonic time(B),ultrasonic power(C),and solid liquid ratio(D)selected as the evaluation factors.The content of scopolamine was used as the evaluation index,and the Box Behnken response surface method was used to optimize the extraction process of scopolamine in Herba Hedyotids Chrysotridhae.Result:The optimal extraction process is ethanol concentration of 74%,extraction time of 45 minutes,ultrasonic power of 250 W,and liquid solid ratio of 25∶1.Under these conditions,the content of scopolamine is 81.97μg/g,which showed no significant difference from the predicted value of 81.41μg/g.Conclusion:The HPLC method established in this experiment can be used for the determination of scopolamine in Herba Hedyotids Chrysotridhae.The optimized extraction process is stable,reliable,simple,and feasible.

关 键 词：HPLC Box Behnken响应面 黄毛耳草 东莨菪内酯 工艺优化 

分 类 号：R284.2[医药卫生—中药学]