半滑舌鳎响应急性高温胁迫的生理变化和相关基因表达  

Physiological Changes and Related Gene Expression of Cynoglossus semilaevis in Response to Acute High Temperature Stress

作　　者：王润 刘洋[2] 杨英明[2] 王婧[1] 张婷婷[1,2] 林梦娇 王磊[2] 陈松林[1,2] WANG Run;LIU Yang;YANG Yingming;WANG Jing;ZHANG Tingting;LIN Mengjiao;WANG Lei;CHEN Songlin(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;State Key Laboratory of Mariculture Biobreeding and Sustainable Goods,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]海水养殖生物育种与可持续产出全国重点实验室中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《渔业科学进展》2024年第4期24-33,共10页Progress in Fishery Sciences

基　　金：国家重点研发计划(2022YFD2400401);中国水产科学研究院基本科研业务费(2022TD20);国家海水鱼产业技术体系半滑舌鳎育种岗位(CARS-47-G03);山东省重点研发计划项目-住鲁院士团队支持项目(2023ZLYS02);山东省泰山学者攀登计划共同资助。

摘　　要：夏季高温会引起半滑舌鳎(Cynoglossus semilaevis)的应激,甚至造成死亡,是工厂化养殖的重要影响因素之一。为探究高温胁迫对半滑舌鳎肝脏氧化损伤及热应激相关基因的影响,本研究选取半滑舌鳎一个全同胞家系为实验对象,通过连续升温达到高温胁迫条件(35℃)后,分别在0、3、6、12和24 h采集肝脏组织,进行苏木精–伊红染色法(HE)和TUNEL染色并观察细胞损伤情况,测定抗氧化酶活性及丙二醛(MDA)含量并检测应激相关基因heatshockprotein familyAmember1A(hspa1a)、heatshockprotein90betafamilymember1(hsp90b1)和dual-specificity phosphatase 1(dusp1)的表达变化。结果显示,急性高温胁迫会造成半滑舌鳎肝脏组织发生明显病理变化并出现细胞凋亡;超氧化物歧化酶(SOD)活性在高温胁迫6 h时显著高于对照组(P<0.05),谷胱甘肽过氧化物酶(GPx)活性在高温胁迫12h时显著高于对照组(P<0.05),过氧化氢酶(CAT)活性在高温胁迫0h时显著高于对照组(P<0.05),MDA含量在高温胁迫24h时显著高于对照组(P<0.05);热休克蛋白基因hspa1a和hsp90b1分别在高温胁迫0 h和3 h时显著上调表达,热应激相关基因dusp1在高温胁迫3h时显著上调表达。综上所述,急性高温胁迫下,半滑舌鳎肝脏发生氧化应激,短期内机体可调动抗氧化系统加速清除活性氧,并激活热应激相关基因表达。该研究可为解析半滑舌鳎对高温胁迫的响应机制、预防夏季高温大规模死亡的发生以及开展耐高温良种的选育等提供参考。The Chinese tongue sole(Cynoglossus semilaevis)belonging to Pleuronectiformes,Cynoglossidae,Cynoglossus,is distributed in the sea areas of Korea,Japan and China.C.semilaevis has limited natural resources and no long-distance migration,which is suitable for the development of aquaculture in coastal areas.After more than ten years of artificial domestication,C.semilaevis has become one of the main mariculture species.Water temperature is an important environmental factor affecting the growth and development of fish.High temperature in summer can cause stress and even death of C.semilaevis,which is one of the important factors affecting factory farming and the promotion in the southern coast.However,the physiological and molecular changes of C.semilaevis in response to acute high temperature stress was still unclear to date.When fish are stimulated by high temperature,it can cause oxidative stress,resulting in a variety of toxic effects.It has been found that there is a significant correlation between high temperature and the activity of antioxidant enzymes in many aquatic animals.Therefore,changes in the activities of antioxidant enzymes can be used as an important indicator when fish are in a state of oxidative stress under high temperature stress.As a tissue related to detoxification metabolism and immunity of fish,liver plays an important role in response to high temperature stress,thus the tissue structure and apoptosis are also important indicators of liver health status after high temperature stress.Heat shock proteins(HSPs)are a class of common biological stress proteins,which have biological functions such as anti-stress,anti-oxidation,and regulation of apoptosis.HSPs are also involved in the resistance of fish to heat stress.Recently,it has been found that zebrafish(Danio rerio)is extremely sensitive to temperature changes after knockout of dual-specificity phosphatase 1(dusp1)gene,and dusp1 has the possibility of maintaining redox homeostasis,so it was speculated that dusp1 gene may plays an important rol

关 键 词：半滑舌鳎 急性高温胁迫 肝脏 细胞凋亡 抗氧化酶 热休克蛋白基因 

分 类 号：S968.1[农业科学—水产养殖]