半滑舌鳎rimoc1基因在雌雄鱼中的差异表达  

Differential Expression of the rimoc1 Gene in Male and Female Chinese Tongue Sole(Cynoglossus semilaevis)

作　　者：孙宇轩 张梦倩 李璐 魏敏 徐文腾 SUN Yuxuan;ZHANG Mengqian;LI Lu;WEI Min;XU Wenteng(Jiangsu Ocean University,Jiangsu Key Laboratory of Marine Biological Resources and Ecological Environment/Jiangsu Key Laboratory of Marine Biotechnology,Lianyungang 222005,China;Yellow Sea Fisheries Research Institute,Ministry of Agriculture and Rural Affairs,Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao Marine Science and Technology Center,Qingdao 266071,China)

机构地区：[1]江苏海洋大学江苏省海洋生物资源与生态环境重点实验室/江苏省海洋生物技术重点实验室,江苏连云港222005 [2]中国水产科学研究院黄海水产研究所青岛海洋科技中心海洋渔业科学与食物产出过程功能实验室,山东青岛266071

出　　处：《渔业科学进展》2024年第4期34-42,共9页Progress in Fishery Sciences

基　　金：国家重点研发计划(2022YFD2400405);中国水产科学研究院黄海水产研究所基本科研业务费(20603022023019);国家自然科学基金(32072955);山东省重点研发计划项目-住鲁院士团队支持项目(2023ZLYS02)共同资助。

摘　　要：动物中普遍存在性别生长二态性,这种现象在海水鱼中更为明显。半滑舌鳎(Cynoglossus semilaevis)性成熟雌鱼体重显著高于雄鱼,雌性个体的生长速度比雄性快2~4倍。本研究旨在探究rimoc1基因在半滑舌鳎雌、雄鱼中的表达差异及与性别和生长的关系。实时定量和原位杂交结果显示,rimoc1在雄性组织中未检测到表达,而在雌性组织中表达量最高的是卵巢和肌肉,在卵巢中表达相对稳定,在孵化后1.5年(1.5yph)和3yph的水平有所增加。在细胞系中通过si RNA干扰敲除rimoc1后,发现生长相关基因igf1、性别分化相关基因sox9b和foxl2的表达水平显著降低,而sox9a的表达升高。启动子活性分析显示,rimoc1启动子可能受到C/EBPdelta、Sox2和c-Jun等转录因子的调控。推测rimoc1可能在半滑舌鳎的性别分化和生长中发挥重要作用,为深入研究半滑舌鳎的性别生长二态性提供了基础。Chinese tongue sole(Cynoglossus semilaevis)is a traditional,nutrient rich and economically valuable fish that is important for mariculture in China.It has small visceral masses,few spines,with tender and tasty flesh.Studies on the sexing mechanism of fish were carried out since the revelation of sexual reversal and hermaphroditism in the eel.Sex is the most common biological phenomenon and there are significant differences in morphology,reproductive strategies,and behavior between females and males of many organisms.Sole is a typical representative of the heterogeneous growth of males and females:adult females are approximately 2–4 times larger than males.This has constrained the sustainable development of the sole culture industry.Why do females grow faster than males?Do sex differentiation-related genes play a role in sex determination and size heteromorphism in sole?Answering these questions has important implications and applications in the genetic improvement and development of sole farming.Rimoc1 encodes a protein in the inner mitochondrial membrane that is involved in regulating the activity of complexⅠof the mitochondrial respiratory chain that affects mitochondrial respiratory function.Rimoc1 interacts with the NDUFAF1 subunit of complexⅠin the inner mitochondrial membrane and enhances its affinity for NADH.This increases the catalytic activity of complexⅠand increases the rate of electron transfer in the mitochondrial respiratory chain.In addition,RIMOC1 interacts with proteins in other mitochondrial respiratory chain complexes(such as complexⅢand complexⅣ)and may play a role in regulating the overall stability and function of the mitochondrial respiratory chain.This study aimed to investigate the expression differences of the rimoc1 gene in male and female Chinese tongue sole and determine its potential relationship with sex and growth.Rimoc1 expression was highest in the ovaries and muscles of females;it was not expressed in male tissues.Interestingly,its expression was relatively stable i

关 键 词：半滑舌鳎 rimoc1 性别生长二态性 表达模式 启动子活性 RNA干扰 

分 类 号：S917.4[农业科学—水产科学]