一株PRRSV-2谱系1.8与1.5重组毒株的基因组特征分析  

Genomic Characterization of a Recombinant Strain of PRRSV-2 between Lineages 1.8 and 1.5

作　　者：杨程 刘野 程宁 王凯月 李欣蕾 孙久英 韩俊平 李文军 王欢欢 邵笑 程雪娇 孙英峰 YANG Cheng;LIU Ye;CHENG Ning;WANG Kaiyue;LI Xinlei;SUN Jiuying;HAN Junping;LI Wenjun;WANG Huanhuan;SHAO Xiao;CHENG Xuejiao;SUN Yingfeng(College of Animal Science and Animal Medicine,Tianjin Agricultural University,Tianjin 300384,China;Tianjin Jinken Animal Husbandry Group Co.,Ltd,Tianjin 300392,China;Tianjin Nongken Kangjia Ecological Breeding Co.,Ltd,Tianjin 300386,China;Tianjin Zhongsheng Challenge Biotechnology Co.,Ltd,Tianjin 300386,China)

机构地区：[1]天津农学院动物科学与动物医学学院,天津300384 [2]天津津垦牧业集团有限公司,天津300392 [3]天津农垦康嘉生态养殖有限公司,天津300386 [4]天津市中升挑战生物科技有限公司,天津300386

出　　处：《畜牧兽医学报》2024年第8期3570-3578,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：天津市科技计划项目(22YFZCSN00100,22ZYCGSN00570,22YDTPJC00420)。

摘　　要：为探究天津地区猪繁殖与呼吸综合征病毒(PRRSV)毒株的流行变异情况。本研究利用猪肺泡巨噬细胞(PAMs)分离培养、有限稀释法纯化和IFA鉴定,从具有严重呼吸症状断奶仔猪群中分离鉴定出一株新的PRRSV流行毒株(命名为TJ-C6),并对其开展了全基因组扩增及其分子特征分析。结果显示,该毒株全基因组全长15011 bp,与NADC30毒株的核苷酸相似性最高(91.7%),为NADC30-like PRRSV(谱系1.8),且其Nsp 2存在131个氨基酸不连续缺失分子特征(“111+1+19 aa”);GP5 N-糖基化位点分析显示存在3个糖基化位点(N32、N43、N50)。重组分析结果显示,该毒株是以NADC30-like(谱系1.8)为骨架,与NADC34-like(谱系1.5)发生重组的PRRSV,其重组区域位于12213~14628 nt(ORF2a~ORF6)。本研究分离鉴定到一株谱系1.8与谱系1.5重组的PRRSV-2毒株,可为天津地区的猪繁殖与呼吸综合征综合防控提供参考。In order to investigate the epidemic variation of porcine reproductive and respiratory syndrome virus(PRRSV)strain in Tianjin area,a new epidemic strain of PRRSV(named TJ-C6)was isolated and identified from weaned piglets with severe respiratory symptoms by means of isolation and culture of porcine alveolar macrophages(PAMs),purification by limited dilution method and identification by IFA.The whole genome amplification and its molecular characteristics were analyzed.The results showed that the whole genome length of this strain was 15011bp,and the nucleotide homology with NADC30 strain was the highest(91.7%),which was NADC30-like PRRSV(lineage 1.8).Nsp2 had 131 discontinuous deletion features("111+1+19aa").GP5 N-glycosylation site analysis showed that there were three glycosylation sites(N32,N43,N50).Recombination analysis showed that this strain was a recombinant PRRSV with NADC30-like(lineage 1.8)as the skeleton and NADC34-like(lineage 1.5),and its recombination region was located in 12213-14628nt(ORF2a-ORF6).In this study,a recombinant PRRSV-2 strain of lineage 1.8 and lineage 1.5 was isolated and identified,which can provide reference for the prevention and control of porcine reproductive and respiratory syndrome in Tianjin.

关 键 词：猪繁殖与呼吸综合征病毒 分离鉴定 基因组特征 重组 

分 类 号：S852.659.6[农业科学—基础兽医学]