禽白血病病毒J亚群感染鸡组织中细胞受体chNHE 1的表达分析  

Analysis of Cellular Receptor chNHE 1 Expression in Chicken Tissues Infected with Avian Leukosis Virus Subgroup J

作　　者：张莉 于蒙蒙 王颖 王素艳 许壮壮 刘鹏 陈运通 祁小乐[2] 李留安 高玉龙[2] ZHANG Li;YU Mengmeng;WANG Ying;WANG Suyan;XU Zhuangzhuang;LIU Peng;CHEN Yuntong;QI Xiaole;LI Liuan;GAO Yulong(Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300392,China;Avian Immunosuppressive Disease Division,State Key Laboratory for Animal Disease Control and Prevention,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,Heilongjiang,China)

机构地区：[1]天津农学院动物科学与动物医学学院、天津市农业动物繁育与健康养殖重点实验室,天津300392 [2]中国农业科学院哈尔滨兽医研究所、动物疫病防控全国重点实验室禽免疫抑制病创新团队,哈尔滨150069

出　　处：《畜牧兽医学报》2024年第8期3631-3639,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金重点项目(32230105);国家肉鸡产业技术体系项目(CARS-41)。

摘　　要：旨在研究鸡钠氢交换蛋白1(chicken sodium hydrogen exchanger isoform 1,chNHE1)在不同类型鸡组织内的表达情况,J亚群禽白血病病毒(subgroup J avian leukosis virus, ALV-J)的组织嗜性及ALV-J感染后组织chNHE1的mRNA变化情况。本研究利用qPCR技术,检测了商品肉鸡、商品蛋鸡和SPF鸡脏器内的chNHE1转录量,ALV-J感染鸡后各组织的病毒载量及组织chNHE1的转录量的变化。结果显示,chNHE1在3种类型鸡的心、肝、脾、肺、肾、盲肠扁桃体、胸腺和法氏囊中均能检测到,但转录量高低存在较大差异,其中,免疫器官内的chNHE1转录量相对较高;SPF鸡接种ALV-J后,心、脾、肾和盲肠扁桃体中的病毒载量相对较高,而肝、肺、胸腺和法氏囊中的病毒载量相对较低;ALV-J感染后,脾、肾和盲肠扁桃体中的chNHE1转录量明显上调,但心中的转录量与空白对照相比无明显变化。本研究分析了3种类型鸡chNHE1的组织转录特点及ALV-J感染对组织内chNHE1转录的影响,该研究结果为探究受体chNHE1的转录与ALV-J的组织嗜性的关系提供重要的理论依据。This study aimed to investigate the expression of chicken sodium hydrogen exchanger isoform 1(chNHE1)in different types of chicken tissues,the tissue tropism of subgroup J avian leukosis virus(ALV-J),and the changes in mRNA of chNHE 1 after ALV-J infection.We used qPCR to detect the transcription of chNHE 1 in the organs of commercial broilers,commercial layers,and SPF chickens,as well as the viral load in various tissues and the changes in chNHE 1 transcription in tissues after ALV-J infection.The results showed that chNHE 1 was transcripted in the heart,liver,spleen,lung,kidney,cecal tonsil,thymus,and bursa in all three types of chickens,but the transcription levels varied greatly,with relatively higher transcription levels in the immune organs.After SPF chickens were infected with ALV-J,the viral load was relatively higher in the heart,spleen,kidney,and cecal tonsils,while it was relatively lower in the liver,lung,thymus,and bursa.The transcription of chNHE 1 in the spleen,kidney,and cecal tonsils was significantly upregulated after ALV-J infection,but there was no significant change in the transcription in the heart compared to the control group.This study analyzed the tissue transcription characteristics of chNHE 1 in three types of chicken and the effect of ALV-J infection on the transcription chNHE 1 expression in tissues,providing an important theoretical basis for exploring the relationship between the expression transcription of the receptor chNHE 1 and the tissue tropism of ALV-J.

关 键 词：J亚群禽白血病病毒 鸡钠氢交换蛋白1 组织表达 转录量变化 

分 类 号：S852.659.3[农业科学—基础兽医学]