蒲公英与木通提取物对断奶仔兔生长性能、肠道健康和药物转运体基因相对表达量的影响  

Effects of Dandelion and Akebia Extract on Growth Performance,Intestinal Health and Relative Expression of Drug Transporter Genes Expression of in Weaned Rabbits

作　　者：陈雨 修子清 MGENI Musa 施屹 张俊秋 蒋小雨 吕景智[1] 孙雅望 CHEN Yu;XIU Ziqing;MGENI Musa;SHI Yi;ZHANG Junqiu;JIANG Xiaoyu;L Jingzhi;SUN Yawang(College of Animal Science and Technology,Southwest University,Chongqing 400715,China;College of Veterinary Medicine,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学动物科学技术学院,重庆400715 [2]西南大学动物医学院,重庆400715

出　　处：《畜牧兽医学报》2024年第8期3725-3739,共15页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：大学生创新创业项目(S202110635182)。

摘　　要：本试验旨在研究饲粮中添加蒲公英、木通提取物对断奶仔兔生长性能、腹泻情况、肠道形态结构、肠黏膜紧密连接蛋白和肝肠药物转运体蛋白基因表达的影响,探究二者联用对提高中药提取物生物利用度的效果。试验选取120只35日龄断奶仔兔,随机分为4个组,每组设置10个重复,每个重复3只兔。试验包括对照组(基础饲粮)、蒲公英组(基础饲粮添加0.5%蒲公英提取物)、木通组(基础饲粮添加0.5%木通提取物)和蒲公英+木通组(基础饲粮分别添加0.5%蒲公英和木通提取物)。试验时间为28 d。试验结果表明:1)第1周蒲公英组和木通组平均日增重显著高于对照组(P<0.05),料重比显著低于对照组(P<0.05)。2)蒲公英组仔兔空肠绒毛高度显著高于木通组(P<0.05)。3)蒲公英+木通组空肠黏膜总黄酮含量显著高于其它各组(P<0.05)。4)蒲公英+木通组闭合小环蛋白1(Z0-1)基因在回肠的相对表达量显著高于蒲公英组和木通组(P<0.05)。5)木通组空肠多药耐药相关蛋白2(MRP-2)、有机阴离子转运多肽2B1(organic anion transporting polypeptide 2B1,OATP2B1)基因的相对表达量显著低于对照组(P<0.05),蒲公英+木通组空肠OATP2B1基因相对表达水平显著低于对照组(P<0.05);蒲公英组和木通组多药耐药相关蛋白3(multidrug resistance-associated protein 3,MRP3)基因在回肠的相对表达量显著低于对照组(P<0.05);蒲公英+木通组乳腺癌耐药相关蛋白(BCRP)、MRP3基因在肝的相对表达量显著低于对照组(P<0.05),木通组和蒲公英+木通组OATP2B1基因在肝的相对表达量显著低于对照组(P<0.05)。综上所述,饲粮中分别添加蒲公英和木通提取物对断奶仔兔的生长性能有所改善,同时添加蒲公英和木通提取物能提高空肠组织总黄酮含量,饲粮中添加蒲公英和木通提取物能调节肝和肠黏膜组织中药物转运体基因表达水平。The objective of this experiment was to investigate the effects of dietary supplemented with dandelion and akebia extract on growth performance,diarrhea,intestinal morphology and structure,total flavonoids in liver and intestine,intestinal mucosa tight junction protein gene expression,drug transporter gene expression in liver and intestine of weaned rabbits,and to explore whether the combination of dandelion and akebia extract can improve the bioavailability of Chinese medicine extract.A hundred and twenty 35-day-old weaned rabbits were randomly divided into 4 groups with 10 replicates per group and 3 rabbits per replicate.Four treatment groups were set up,groups including control group(basic diet),dandelion group(basic diet+0.5%dandelion extract),akebia group(basic diet+0.5%akebia extract),and dandelion+akebia group(basic diet+0.5%dandelion extract+0.5%akebia extract).The trial lasted for 28 days.The results were showed as follows:1)At the first week of the experiment,compared with control group,the ADG in dandelion group and akebia group was significantly increased(P<0.05),the value of F/G was significantly decreased(P<0.05).2)The villus height of jejunum in dandelion group was significantly higher than that in akebia group(P<0.05).3)The content of total flavonoids in jejunal mucosa of dandelion+akebia group was significantly higher than other groups(P<0.05).4)The relative expression level of zonula occluden 1(ZO-1)gene in ileum of group dandelion+akebia group was significantly higher than that of dandelion group and akebia group(P<0.05).5)The relative expression levels of multidrug resistance-associated protein 2(MRP2)and organic anion transporting polypeptide 2B1(OATP2 B1)genes in jejunum of akebia group was significantly lower than those of control group(P<0.05),and OATP2B1 gene the relative expression level of OATP2 B1 in jejunum of dandelion+akebia group was significantly lower than control group(P<0.05).The relative expression of multidrug resistance-associated protein 3(MRP3)gene in ileum of dandelion gr

关 键 词：蒲公英 木通 断奶仔兔 生长性能 肠道健康 总黄酮 药物转运体 

分 类 号：S816.75[农业科学—饲料科学]