RpUGT344J7 is involved in the reproduction switch of Rhopalosiphum padi with holocyclic life cycle  

作　　者：Suji Wang Wenjie Huang Mengtian Li Ni Wang Xi Liu Maohua Chen Xiong Peng 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas,Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education,Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi Province,China

出　　处：《Insect Science》2024年第4期1073-1089,共17页昆虫科学（英文版）

基　　金：funded by the National Natural Science Foundation of China(grant nos.32272541,31901878,and 32172407);the National Key R&D Program of China(grant no.2022YFD1400900).

摘　　要：Many aphid species exhibit both cyclical parthenogenesis(CP)and the obligate parthenogenesis(OP)life history,which are genetically determined.In CP aphid lineages,the parthenogenetic individuals can switch from asexual to sexual reproduction quickly in response to environmental factors such as changes in photoperiod and temperature.However,the OP aphid lineages do not undergo sexual reproduction under any conditions.So far,mechanisms underlying the reproduction switch in CP aphids have not been fully elucidated.Rhopalosiphum padi,a serious worldwide insect pest of wheat,has both CP and OP lineages.Uridine diphosphate-glycosyltransferases(UGTs)are enzymes that participate in the metabolic detoxification of xenobiotics.Here,we identified 43 RpUGT genes from R.padi genome and transcriptome sequences,and found that:(1)the UGT content of the CP lineage was significantly higher than that in the OP lineage at the key time points when CP lineage mainly produce virginoparae,gynoparae,and males under inducing condition,while there were no significant difference under normal conditions;(2)RpUGT344J7 gene was highly expressed during the time points when CP lineages produce gynopara and males;(3)the critical time points for CP lineages to produce virginoparaee,gynoparae,and males were affected when the CP lineages were injected with dsRpUGT344J7;(4)the knockdown of RpUGT344J7 caused a significant reduction in the total number of virginoparae,gynoparae,and males in the offspring under inducing condition.The findings contribute to our understanding of the molecular mechanisms underlying the quick shift from asexual to sexual reproduction in aphid species.

关 键 词：bird cherry-oat aphid cyclical parthenogenesis obligate parthenogenesis reproduction switch uridine diphosphate-glycosyltransferases(UGTs) 

分 类 号：Q96[生物学—昆虫学]